Clinic of Obstetrics and Gynecology, University of Health Sciences, İstanbul Training and Research Hospital, İstanbul, Turkey.
Department of Obstetrics and Gynecology, İzmir Katip Çelebi University School of Medicine, İzmir, Turkey.
Balkan Med J. 2017 Sep 29;34(5):444-449. doi: 10.4274/balkanmedj.2016.1386. Epub 2017 Apr 13.
Apigenin is a plant-derived compound belonging to the flavone class, which possess antioxidant, free-radical-scavenging and anti-inflammatory properties.
To address the effects of apigenin on serum anti-mullerian hormone levels, tissue oxidative stress parameters and histopathological changes in ovarian ischemia/reperfusion injury.
Animal experiment.
Twenty-eight female Wistar albino rats were randomly separated into four sections: Sham operation (group 1), ischemia/reperfusion plus saline (group 2), ischemia/reperfusion plus dimethyl sulfoxide (group 3) and ischemia/reperfusion plus apigenin (group 4). In all ischemia/reperfusion groups, a bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion. A single dose of 15 mg/kg apigenin was given intraperitoneally 60 min before reperfusion in group 4. After 3-h of reperfusion, both ovaries were removed, and blood samples were collected. The main outcome measures were serum anti-mullerian hormone levels, ovarian tissue malondialdehyde, total nitric oxide, Cu/Zn superoxide dismutase, catalase and glutathione levels and histopathological damage scores.
The ovarian tissue nitric oxide level was significantly lower, and the glutathione level was significantly higher in group 4 compared with groups 2 and 3. There was no significant difference in anti-mullerian hormone levels among the three ischemia/reperfusion groups. The histopathological damage score was lower in group 4 than in groups 2 and 3 (p>0.05).
Administration of apigenin has no significant protective effect on ovarian reserve and tissue damage in ovarian ischemia/reperfusion injury.
芹菜素是一种植物衍生的化合物，属于黄酮类，具有抗氧化、清除自由基和抗炎的特性。
研究芹菜素对卵巢缺血/再灌注损伤中血清抗苗勒管激素水平、组织氧化应激参数和组织病理学变化的影响。
动物实验。
将 28 只雌性 Wistar 白化大鼠随机分为四组：假手术（第 1 组）、缺血/再灌注加生理盐水（第 2 组）、缺血/再灌注加二甲基亚砜（第 3 组）和缺血/再灌注加芹菜素（第 4 组）。在所有缺血/再灌注组中，进行双侧附件 3 小时的缺血，然后再灌注 3 小时。第 4 组在再灌注前 60 分钟给予 15mg/kg 芹菜素单次腹腔内注射。再灌注 3 小时后，取出双侧卵巢，采集血样。主要观察指标为血清抗苗勒管激素水平、卵巢组织丙二醛、总一氧化氮、Cu/Zn 超氧化物歧化酶、过氧化氢酶和谷胱甘肽水平以及组织病理学损伤评分。
与第 2 组和第 3 组相比，第 4 组卵巢组织一氧化氮水平显著降低，谷胱甘肽水平显著升高。三组缺血/再灌注组的抗苗勒管激素水平无显著差异。第 4 组的组织病理学损伤评分低于第 2 组和第 3 组（p>0.05）。
在卵巢缺血/再灌注损伤中，给予芹菜素对卵巢储备和组织损伤没有显著的保护作用。
