Faculty of Medicine, Department of Obstetrics and Gynecology, University of Inonu, 44280 Malatya, Turkey.
Faculty of Medicine, Department of Medical Pharmacology, University of Inonu, 44280 Malatya, Turkey.
Biomed Res Int. 2018 May 14;2018:5421308. doi: 10.1155/2018/5421308. eCollection 2018.
To evaluate the protective effects of glycyrrhetinic acid (GA) and chrysin (CH) on experimental ischemia-reperfusion (I/R) injury in rat ovaries using tissue oxidative stress marker levels, hormone levels, and histopathological scores.
Sixty healthy rats were randomly divided into six equal groups: control, I/R, I/R + CH (50 mg/kg/day), I/R + GA (100 mg/kg/day), CH (50 mg/kg/day), and GA (100 mg/kg/day). Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after CH and GA treatment.
The antioxidant defense system parameters were significantly higher in the ovarian tissues of the I/R + CH and I/R + GA groups than in those of the I/R group. Serum follicle-stimulating hormone levels were significantly reduced, and serum anti-Müllerian hormone levels were significantly increased in rats treated with CH and GA compared with those in the I/R group. Additionally, the histopathological scores of the I/R + CH and I/R + GA groups were significantly improved compared with those of the I/R group.
The significant improvements in tissue oxidative stress parameters, serum hormone levels, and histological scores observed in this study indicate that treatment with CH or GA may be a conservative approach to prevent I/R injury in adnexal torsion cases after the ovarian detorsion procedure.
通过评估组织氧化应激标志物水平、激素水平和组织病理学评分，研究甘草次酸（GA）和白杨素（CH）对大鼠卵巢实验性缺血再灌注（I/R）损伤的保护作用。
将 60 只健康大鼠随机分为 6 组：对照组、I/R 组、I/R+CH（50mg/kg/天）组、I/R+GA（100mg/kg/天）组、CH（50mg/kg/天）组和 GA（100mg/kg/天）组。在 CH 和 GA 治疗 14 天后，对血液和组织样本进行生化、激素和组织病理学评估。
与 I/R 组相比，I/R+CH 和 I/R+GA 组卵巢组织的抗氧化防御系统参数显著升高。与 I/R 组相比，CH 和 GA 治疗组大鼠血清卵泡刺激素水平显著降低，血清抗苗勒管激素水平显著升高。此外，与 I/R 组相比，I/R+CH 和 I/R+GA 组的组织病理学评分显著改善。
本研究中观察到的组织氧化应激参数、血清激素水平和组织学评分的显著改善表明，在卵巢复位手术后，用 CH 或 GA 治疗可能是预防附件扭转病例 I/R 损伤的一种保守方法。
