School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
Carbohydr Res. 2009 Dec 14;344(18):2512-8. doi: 10.1016/j.carres.2009.08.042. Epub 2009 Sep 3.
A water-soluble glucan (RCP-1) was prepared from the roots of Rubus crataegifolius Bge. by extraction with hot-water, deproteination by Sevag reagent, alpha-amylase treatment and ultrafiltration. RCP-1 consisted of only glucose, and its molecular weight was determined to be approximately 7KD by high performance gel permeation chromatography (HPGPC). Fourier transform infra-red spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR), methylation and periodate oxidation analyses indicated that RCP-1 was an alpha-d-glucan. Its main chains were composed of (1-->4)- and (1-->6)-linked alpha-glucopyranosyls, and side chains were single alpha-glucopyranosyl residues attached to the O-6 of glucosyls in the main chains. RCP-1 could increase both cytotoxic activity against B16 melanoma cells and the production of nitric oxide (NO) of macrophages in vitro. Furthermore, in vivo bioassay tests indicated that RCP-1 could remarkably enhance T and B lymphocyte proliferations, augment the phagocytosis of macrophages and increase the tumour necrosis factor-alpha (TNF-alpha) levels in serum.
从悬钩子属植物根(Rubus crataegifolius Bge.)中提取水溶性葡聚糖（RCP-1），采用热水提取、Sevag 试剂脱蛋白、α-淀粉酶处理和超滤。RCP-1仅由葡萄糖组成，其分子量通过高效凝胶渗透色谱（HPGPC）确定为约 7KD。傅里叶变换红外光谱（FT-IR）、核磁共振光谱（NMR）、甲基化和过碘酸盐氧化分析表明，RCP-1 是一种α-D-葡聚糖。其主链由（1→4）-和（1→6）-连接的α-葡萄糖吡喃基组成，侧链是单α-葡萄糖吡喃基残基，连接在主链中葡萄糖基的 O-6 上。RCP-1 可以体外增加对 B16 黑色素瘤细胞的细胞毒性活性和巨噬细胞一氧化氮（NO）的产生。此外，体内生物测定试验表明，RCP-1 可以显著增强 T 和 B 淋巴细胞的增殖，增强巨噬细胞的吞噬作用，并增加血清中肿瘤坏死因子-α（TNF-α）的水平。
