Awadasseid Annoor, Hou Jie, Gamallat Yaser, Xueqi Shang, Eugene Kuugbee D, Musa Hago Ahmed, Bamba Djibril, Meyiah Abdo, Gift Chiwala, Xin Yi
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Department of Biochemistry and Molecular Biology, Northeast Normal University, Changchun, P.R. China.
PLoS One. 2017 Feb 8;12(2):e0171270. doi: 10.1371/journal.pone.0171270. eCollection 2017.
Cancer is one of the most common causes of deaths worldwide. Herein, we report an efficient natural anticancer glucan (CVG) extracted from Coriolus Versicolar (CV). CVG was extracted by the hot water extraction method followed by ethanol precipitation and purified using gas exclusion chromatography. Structural analysis revealed that CVG has a linear α-glucan chain composed of only (1→ 6)-α-D-Glcp. The antitumor activity of CVG on Sarcoma-180 cells was investigated in vitro and in vivo. Mice were treated with three doses of CVG (40, 100, 200 mg/kg body weight) for 9 days. Tumor weight, relative spleen, thymus weight, and lymphocyte proliferation were studied. A significant increase (P< 0.01) in relative spleen and thymus weight and a decrease (P< 0.01) in tumor weight at the doses of 100 and 200 mg/kg were observed. The results obtained demonstrate CVG has antitumor activity towards Sarcoma-180 cells by its immunomodulation activity.
癌症是全球最常见的死亡原因之一。在此，我们报告一种从云芝中提取的高效天然抗癌葡聚糖（CVG）。CVG通过热水提取法提取，然后乙醇沉淀，并使用排阻色谱法纯化。结构分析表明，CVG具有仅由（1→6）-α-D-葡萄糖组成的线性α-葡聚糖链。在体外和体内研究了CVG对肉瘤180细胞的抗肿瘤活性。用三种剂量的CVG（40、100、200mg/kg体重）处理小鼠9天。研究了肿瘤重量、相对脾脏、胸腺重量和淋巴细胞增殖。在100和200mg/kg剂量下，观察到相对脾脏和胸腺重量显著增加（P<0.01），肿瘤重量降低（P<0.01）。所得结果表明，CVG通过其免疫调节活性对肉瘤180细胞具有抗肿瘤活性。
