Norwegian Institute for Water Research, Oslo, Norway.
Aquat Toxicol. 2010 Jan 21;96(1):44-52. doi: 10.1016/j.aquatox.2009.09.016. Epub 2009 Oct 1.
The use of nanomaterials is rapidly increasing, while little is known about their possible ecotoxicological effects. This work investigates the toxic effects of silver (Ag) and gold (Au) nanoparticles on rainbow trout hepatocytes. In addition to toxicity assessment the particles were characterized by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). Hepatocyte primary cultures were exposed to Au and Ag nanoparticles, with and without dissolved organic carbon (DOC), as well as HAuCl(4) and AgNO(3) as ionic solutions at concentrations up to 17.4mg/L and 19mg/L, respectively. Ag and Au particles were within the small nanometer size range when dispersed in pure water. In media with higher ionic strength and DOC, particles tended to agglomerate. Cytotoxicity assessments showed that Ag nanoparticles caused a significant reduction in membrane integrity and cellular metabolic activity in a concentration-dependent manner. Au nanoparticles caused a threefold elevation of ROS levels, but no cytotoxicity occurred at concentrations tested. The addition of DOC did not alter the particles potency of cytotoxicity or ROS induction capacity. The current study shows that Ag and Au nanoparticles have adverse effects on rainbow trout hepatocytes at low mg/L concentrations.
纳米材料的使用正在迅速增加，而人们对其可能的生态毒理学影响知之甚少。本研究调查了银（Ag）和金（Au）纳米颗粒对虹鳟鱼肝细胞的毒性作用。除了毒性评估外，还通过透射电子显微镜（TEM）和纳米颗粒跟踪分析（NTA）对颗粒进行了表征。在高达 17.4mg/L 和 19mg/L 的浓度下，将含有和不含有溶解有机碳（DOC）的 Au 和 Ag 纳米颗粒以及 HAuCl4 和 AgNO3 作为离子溶液，与虹鳟鱼原代肝细胞培养物一起暴露。Ag 和 Au 颗粒在纯水中分散时处于小纳米尺寸范围内。在具有更高离子强度和 DOC 的介质中，颗粒倾向于聚集。细胞毒性评估表明，Ag 纳米颗粒以浓度依赖的方式导致细胞膜完整性和细胞代谢活性显著降低。Au 纳米颗粒导致 ROS 水平升高三倍，但在测试浓度下没有发生细胞毒性。DOC 的添加并没有改变颗粒的细胞毒性或 ROS 诱导能力。本研究表明，Ag 和 Au 纳米颗粒在低 mg/L 浓度下对虹鳟鱼肝细胞具有不良影响。
