Li Sitian, Wang Xiu, Wang Wanxia, Zhang Zhongxing, Wang Xingbin, Zhang Qingxia, Wang Yanxiu
College of Horticulture, Gansu Agricultural University, Lanzhou, 730070 China.
College of Agriculture and Forestry Technology, Longdong University, Qingyang, 745000 China.
Physiol Mol Biol Plants. 2024 Apr;30(4):633-645. doi: 10.1007/s12298-024-01444-7. Epub 2024 Apr 6.
ALDH (Aldehyde dehydrogenase), as an enzyme that encodes the dehydroxidization of aldehydes into corresponding carboxylic acids, played an important role inregulating gene expression in response to many kinds of biotic and abiotic stress, including saline-alkali stress. Saline-alkali stress was a common stress that seriously affected plant growth and productivity. Saline-alkali soil contained the characteristics of high salinity and high pH value, which could cause comprehensive damage such as osmotic stress, ion toxicity, high pH, and HCO/CO stress. In our study, 18 genes were identified in sweet cherry genome, and their gene structures, phylogenetic analysis, chromosome localization, and promoter -acting elements were analyzed. Quantitative real-time PCR confirmed that exhibited the highest expression compared to other members under saline-alkali stress. Subsequently, it was isolated from , and transgenic was successfully obtained. Compared with wild type, transgenic plants grew better under saline-alkali stress and showed higher chlorophyll content, Superoxide dismutase (SOD), Peroxidase (POD) and Catalase (CAT) enzyme activities, which indicated that they had strong resistance to stress. These results indicated that improved the resistance of sweet cherries to saline-alkali stress, which in turn improved quality and yields.
The online version contains supplementary material available at 10.1007/s12298-024-01444-7.
醛脱氢酶（ALDH）作为一种将醛类氧化为相应羧酸的酶，在响应多种生物和非生物胁迫（包括盐碱胁迫）时调节基因表达中发挥着重要作用。盐碱胁迫是一种严重影响植物生长和生产力的常见胁迫。盐碱土具有高盐分和高pH值的特点，可导致渗透胁迫、离子毒性、高pH值和HCO/CO胁迫等综合损害。在我们的研究中，在甜樱桃基因组中鉴定出18个基因，并对它们的基因结构、系统发育分析、染色体定位和启动子作用元件进行了分析。实时定量PCR证实，与盐碱胁迫下的其他成员相比，其表达量最高。随后，从 中分离出它，并成功获得了转基因 。与野生型相比，转基因 植物在盐碱胁迫下生长更好，叶绿素含量更高，超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性更高，这表明它们具有较强的胁迫抗性。这些结果表明， 提高了甜樱桃对盐碱胁迫的抗性，进而提高了品质和产量。
在线版本包含可在10.1007/s12298-024-01444-7获取的补充材料。
