转移性嗜铬细胞瘤的动物模型：通过MRI和PET进行评估
Animal model of metastatic pheochromocytoma: evaluation by MRI and PET.
作者信息
Martiniova L, Lai E W, Thomasson D, Kiesewetter D O, Seidel J, Merino M J, Kvetnansky R, Pacak K
机构信息
Reproductive and Adult Endocrinology Program, Eunice Kennedy Shriver National Institutes of Child Health and Human Development, Bethesda, MD, 20892, USA.
出版信息
Endocr Regul. 2009 Apr;43(2):59-64.
OBJECTIVE
The development of metastatic pheochromocytoma animal model provides a unique opportunity to study the physiology of these rare tumors and to evaluate experimental treatments. Here, we describe the use of small animal imaging techniques to detect, localize and characterize metastatic lesions in nude mice.
METHODS
Small animal positron emission tomography (PET) imaging and magnetic resonance imaging (MRI) were used to detect metastatic lesions in nude mice following intravenous injection of mouse pheochromocytoma cells. [18F]-6-fluoro-dopamine ([18F]-DA) and [18F]-L-6-fluoro-3,4-dihydroxyphenylalanine, which are commonly used for localization of pheochromocytoma lesions in clinical practice, were selected as radiotracers to monitor metastatic lesions by PET.
RESULTS
MRI was able to detect liver lesions as small as 0.5mm in diameter. Small animal PET imaging using [18F]-DA and [18F]-DOPA detected liver, adrenal gland, and ovarian lesions.
CONCLUSION
We conclude that MRI is a valuable technique for tumor growth monitoring from very early to late stages of tumor progression and that animal PET confirmed localization of metastatic pheochromocytoma in liver with both radiotracers.
目的
转移性嗜铬细胞瘤动物模型的建立为研究这些罕见肿瘤的生理学以及评估实验性治疗提供了独特的机会。在此，我们描述了使用小动物成像技术来检测、定位和表征裸鼠体内的转移性病变。
方法
在静脉注射小鼠嗜铬细胞瘤细胞后，使用小动物正电子发射断层扫描（PET）成像和磁共振成像（MRI）来检测裸鼠体内的转移性病变。选择临床实践中常用于嗜铬细胞瘤病变定位的[18F]-6-氟多巴胺（[18F]-DA）和[18F]-L-6-氟-3,4-二羟基苯丙氨酸作为放射性示踪剂，通过PET监测转移性病变。
结果
MRI能够检测到直径小至0.5毫米的肝脏病变。使用[18F]-DA和[18F]-多巴的小动物PET成像检测到了肝脏、肾上腺和卵巢病变。
结论
我们得出结论，MRI是一种从肿瘤进展的极早期到晚期监测肿瘤生长的有价值的技术，并且动物PET用两种放射性示踪剂均证实了转移性嗜铬细胞瘤在肝脏中的定位。
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