Aliaga Antonio, Rousseau Jacques A, Cadorette Jules, Croteau Etienne, van Lier Johan E, Lecomte Roger, Bénard François
Sherbrooke Molecular Imaging Center, Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada.
Mol Imaging Biol. 2007 May-Jun;9(3):144-50. doi: 10.1007/s11307-007-0091-6.
We used small animal positron emission tomography (PET) imaging to monitor the time-course of tumor metabolic response to hormone and chemotherapy in a murine model of hormone-sensitive breast cancer.
Estrogen receptor positive murine mammary carcinomas were inoculated in Balb/c mice. Small animal PET imaging using 2-deoxy-2-[F-18]fluoro-D: -glucose (FDG) was used to assess tumor metabolic activity. Imaging was done before and at days 1, 7, and 14 after the administration of doxorubicin, methotrexate, letrozole, or placebo. The tumor uptake of FDG was calculated from a region-of-interest drawn around the tumor.
All treatments resulted in a decrease in tumor growth rate and end volume compared to untreated control. FDG uptake was also markedly decreased after treatment although a flare reaction was observed on PET at day 7, the intensity of which varied according to the treatment modality.
PET imaging is sensitive to detect early changes associated with therapy in murine breast cancer models. A flare reaction was observed 7 days after the initiation of therapy.
我们使用小动物正电子发射断层扫描（PET）成像技术，在激素敏感性乳腺癌小鼠模型中监测肿瘤对激素和化疗的代谢反应时间进程。
将雌激素受体阳性的小鼠乳腺癌接种到Balb/c小鼠体内。使用2-脱氧-2-[F-18]氟-D-葡萄糖（FDG）进行小动物PET成像，以评估肿瘤代谢活性。在给予阿霉素、甲氨蝶呤、来曲唑或安慰剂之前以及给药后第1、7和14天进行成像。FDG的肿瘤摄取量通过在肿瘤周围绘制感兴趣区域来计算。
与未治疗的对照组相比，所有治疗均导致肿瘤生长速率和最终体积降低。治疗后FDG摄取也明显降低，尽管在第7天PET上观察到了炎症反应，其强度因治疗方式而异。
PET成像对检测小鼠乳腺癌模型中与治疗相关的早期变化敏感。在治疗开始7天后观察到炎症反应。
