Da Silva Newton Soares, Potrich Juliana W
Laboratório de Biologia Celular & Tecidual, Instituto de Pesquisa & Desenvolvimento, UNIVAP, São José dos Campos, Brazil.
Photomed Laser Surg. 2010 Jun;28(3):431-4. doi: 10.1089/pho.2008.2410.
The aim of this study was to determine the influence of laser irradiation on enzyme activity.
Enzymes are catalysts of extraordinary efficiency, able to accelerate reactions by manifold. Enzyme laser light activation is currently a fast-growing field and a large number of studies have been produced.
Liquid CNPG amylase and control serum (Qualitrol 1H) were used in the experiments. Laboratory analysis of alpha-amylase was performed on two sample groups: (i) E + S and (ii) E + S + L, in six repetitions per irradiation dose. Group 2 was irradiated with gallium-aluminum-arsenide (GaAlAs) 904 nm at doses of 0.01, 0.1, 0.5, and 1 J/cm(2). Enzyme activity was read using a spectrophotometer equipped with a thermostatic chamber capable of precise absorbance measurement at 405 nm.
The results were analyzed with the Student's t-test, and the percentage of enzyme activity was determined. Photomodulation of alpha-amylase activity by GaAlAs laser was analyzed following irradiation with different doses. Irradiation doses from 0.01 to 1 J/cm(2) led to differences in enzyme activity: 0.01 J/cm(2) (0.10%), 0.1 J/cm(2) (13.44%), 0.5 J/cm(2) (12.57%), and 1 J/cm(2) (-6.10%).
Irradiation doses of 0.1 J/cm(2) and 0.5 J/cm(2) led to statistically significant increases in enzyme activity in comparison to the control. The similar curves of the effects of temperature and pH on enzymatic activity observed in this study suggest that laser irradiation also possess an optimum dose to modulate the enzymatic activity. That is, enzymes have an optimum laser dose (or range) at which their activity is maximal, whereas at higher or lower doses activity decreases.
本研究旨在确定激光照射对酶活性的影响。
酶是效率极高的催化剂，能够大幅加速反应。酶的激光光激活目前是一个快速发展的领域，并且已经产生了大量的研究。
实验中使用了液体CNPG淀粉酶和对照血清（Qualitrol 1H）。对两个样本组进行了α-淀粉酶的实验室分析：（i）E + S组和（ii）E + S + L组，每个照射剂量重复6次。第2组用波长904 nm的砷化镓铝（GaAlAs）激光以0.01、0.1、0.5和1 J/cm²的剂量进行照射。使用配备能够在405 nm处精确测量吸光度的恒温箱的分光光度计读取酶活性。
采用学生t检验分析结果，并确定酶活性百分比。分析了不同剂量照射后GaAlAs激光对α-淀粉酶活性的光调制作用。0.01至1 J/cm²的照射剂量导致酶活性出现差异：0.01 J/cm²（0.10%）、0.1 J/cm²（13.44%）、0.5 J/cm²（12.57%）和1 J/cm²（-6.10%）。
与对照组相比，0.1 J/cm²和0.5 J/cm²的照射剂量导致酶活性有统计学意义的增加。本研究中观察到的温度和pH对酶活性影响的相似曲线表明，激光照射也存在调节酶活性的最佳剂量。也就是说，酶有一个最佳激光剂量（或范围），在此剂量下其活性最大，而在更高或更低剂量下活性会降低。
