Mirmiranpour Hossein, Shmas Nosrati Fatemeh, Sobhai Seyed Omid, Nazifi Takantape Sina, Amjadi Ahmad
Endocrinology and Metabolism Research Center (EMRC), Valiasr Hospital, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
Laser and medical Physics lab, Department of Physics, Sharif University of Technology, Tehran, Iran.
J Lasers Med Sci. 2018 Summer;9(3):212-218. doi: 10.15171/jlms.2018.38. Epub 2018 Jul 28.
The aim of this work is to evaluate the effect of low-level laser irradiation (LLLI), by lasers with different wavelengths, on glycated catalase enzyme in vitro experimentally. This is done by measuring the activity and structure properties of glycated catalase enzyme. The structure properties were evaluated with circular dichroism (CD) and fluoroscopy methods. Three continuous wave (CW) lasers in the visible spectrum (λ =450, 530, 638 nm) and a 100-ns pulsed laser in the infrared spectrum (λ =905 nm) were chosen for comparison. For the infrared laser, same effects have been investigated for different energy doses. The effect of photon energy (hυ) at different wavelengths was measured on activity, CD, and fluoroscopy properties of catalase, and compared with the control group (samples without irradiation). The energy intensity of laser should not exceed 0.1 J/cm . Experiments were performed on glycated catalase between 2 to 16 weeks after glycation of catalase. The LLLI effect was also investigated on the samples, by comparing the catalase activity, CD and fluoroscopy for different wavelengths. Our results indicated, the decrease in catalase activity as a function of glycation time (weeks) for all samples, and a slight increase on its activity by different laser wavelengths irradiation for any fixed period of glycation time. Finally, the catalase activity has been increased as the laser's photon energy (hυ) intensified. More specifically, the blue laser (λ =450 nm) had the most and the red laser (λ =638 nm) had the least effect, and the green laser (λ =530 nm) had the medium effect on catalase activity as well. Furthermore, pulsed laser had an additional effect by increasing energy dosage. As we expected in all experiments, an increase in the catalase activity was coincident with a decrease in the catalase fluoroscopy and CD parameters.
这项工作的目的是通过实验在体外评估不同波长的激光进行低强度激光照射（LLLI）对糖化过氧化氢酶的影响。这是通过测量糖化过氧化氢酶的活性和结构特性来完成的。结构特性用圆二色性（CD）和荧光法进行评估。选择了三个可见光谱中的连续波（CW）激光（λ = 450、530、638 nm）和一个红外光谱中的100纳秒脉冲激光（λ = 905 nm）进行比较。对于红外激光，研究了不同能量剂量下的相同效果。测量了不同波长下光子能量（hυ）对过氧化氢酶活性、CD和荧光特性的影响，并与对照组（未照射的样品）进行比较。激光的能量强度不应超过0.1 J/cm²。实验在过氧化氢酶糖化后2至16周的糖化过氧化氢酶上进行。还通过比较不同波长下的过氧化氢酶活性、CD和荧光，研究了样品上的LLLI效应。我们的结果表明，所有样品的过氧化氢酶活性随糖化时间（周）而降低，并且在任何固定的糖化时间内，不同激光波长照射都会使其活性略有增加。最后，过氧化氢酶活性随着激光光子能量（hυ）的增强而增加。更具体地说，蓝光激光（λ = 450 nm）的影响最大，红光激光（λ = 638 nm）的影响最小，绿光激光（λ = 530 nm）对过氧化氢酶活性的影响也处于中等水平。此外，脉冲激光通过增加能量剂量有额外的效果。正如我们在所有实验中所预期的，过氧化氢酶活性的增加与过氧化氢酶荧光和CD参数的降低同时出现。
