Ferreira Marcos Paulo Pinheiro, Ferrari Raquel Agnelli Mesquita, Gravalos Erick Diomedesse, Martins Manoela Domingues, Bussadori Sandra Kalil, Gonzalez Daniela Aparecida Biasotto, Fernandes Kristianne Porta Santos
Rehabilitation Sciences Department, Nove de Julho University, São Paulo, SP, Brazil.
Photomed Laser Surg. 2009 Dec;27(6):901-6. doi: 10.1089/pho.2008.2427.
To evaluate the effect of phototherapy on the viability of cultured C2C12 myoblasts under different nutritional conditions (muscle injury model) using low-energy gallium-aluminum-arsenide (GaAlAs) and aluminium-gallium-indium-phosphide (InGaAlP) lasers with different wavelengths and powers.
The beneficial effects of phototherapy using low-energy lasers depend on irradiation parameters and type of laser used, but there are no data in the literature on C2C12 myoblasts proliferation after phototherapy with GaAlAs and InGaAlP lasers.
A C2C12 cell line cultured in regular (10% fetal bovine serum, FBS) and nutrient-deficient (5% FBS) media were irradiated with low-energy GaAlAs (660 nm) and InGaAlP (780 nm) lasers with energy densities of 3.8, 6.3, and 10 J/cm2, and 3.8, 10, and 17.5 J/cm2, respectively. Cell proliferation was assessed indirectly 24 h after irradiation by measuring the mitochondrial activity and using the crystal violet assay.
There were no significant differences in cell viability between laser-treated myoblasts and control cultures for all tested parameters after 24 h of cell culture, although cell cultures grown in regular nutrient medium supplemented with 10% FBS exhibited higher growth rates than cultures, irradiated or not, grown in nutrient-deficient medium.
Laser phototherapy did not improve C2C12 viability under regular or nutrient-deficient (muscle injury model) conditions using the above parameters.
使用不同波长和功率的低能量砷化镓铝（GaAlAs）和磷化铝镓铟（InGaAlP）激光，评估光疗对不同营养条件下（肌肉损伤模型）培养的C2C12成肌细胞活力的影响。
低能量激光光疗的有益效果取决于照射参数和所用激光的类型，但文献中尚无关于用GaAlAs和InGaAlP激光进行光疗后C2C12成肌细胞增殖的数据。
将在常规（10%胎牛血清，FBS）和营养缺乏（5%FBS）培养基中培养的C2C12细胞系，分别用能量密度为3.8、6.3和10 J/cm²的低能量GaAlAs（660 nm）激光和能量密度为3.8、10和17.5 J/cm²的InGaAlP（780 nm）激光照射。照射24小时后，通过测量线粒体活性和使用结晶紫测定法间接评估细胞增殖。
细胞培养24小时后，对于所有测试参数，激光处理的成肌细胞与对照培养物之间的细胞活力没有显著差异，尽管在补充有10%FBS的常规营养培养基中生长的细胞培养物比在营养缺乏培养基中生长的照射或未照射的培养物表现出更高的生长速率。
使用上述参数，在常规或营养缺乏（肌肉损伤模型）条件下，激光光疗并未提高C2C12的活力。
