CTX-M和TEM是从实体器官移植受者中分离出的粘质沙雷氏菌中主要的超广谱β-内酰胺酶类型。
CTX-M and TEM as predominant types of extended spectrum beta-lactamases among Serratia marcescens isolated from solid organ recipients.
作者信息
Mlynarczyk A, Szymanek K, Sawicka-Grzelak A, Pazik J, Buczkowska T, Durlik M, Lagiewska B, Pacholczyk M, Chmura A, Paczek L, Mlynarczyk G
机构信息
Department of Medical Microbiology, The Medical University of Warsaw, 02-004 Warsaw, Poland.
出版信息
Transplant Proc. 2009 Oct;41(8):3253-5. doi: 10.1016/j.transproceed.2009.08.011.
BACKGROUND
Serratia marcescens is an important pathogen in hospital infections since organisms resistant to multiple antimicrobials pose a special threat particularly among transplant patients. The aim of this work was to assess the number of strains producing beta-lactamases with extended spectrum (ESBL) among S. marcescens isolated from our patients.
MATERIALS AND METHODS
We investigated S. marcescens isolated from 2005 to 2008 for ESBL. The phenotype methods were applied and additionally we chose strains for polymerase chain reactions using primers for the most popular types of ESBL.
RESULTS
Over the investigated time, 257 patients were infected with S. marcescens with 188 (73%) displaying an ESBL-positive phenotype. A Molecular analysis showed that most of them produced both CTX-M and TEM beta-lactamases. In the last year, the percentage of ESBL-producing strains decreased, but also in the last year, we isolated S. marcescens resistant to carbapenems from three patients.
CONCLUSIONS
The CTX-M type of ESBL predominated among ESBLs produced by strains of S. marcescens. The appearance of strains resistant to carbapenems is alarming.
背景
粘质沙雷氏菌是医院感染中的一种重要病原体，因为对多种抗菌药物耐药的菌株构成了特殊威胁，尤其是在移植患者中。这项工作的目的是评估从我们的患者中分离出的粘质沙雷氏菌中产生超广谱β-内酰胺酶（ESBL）的菌株数量。
材料与方法
我们调查了2005年至2008年分离出的粘质沙雷氏菌的ESBL情况。应用了表型方法，此外，我们选择菌株使用针对最常见类型ESBL的引物进行聚合酶链反应。
结果
在调查期间，257名患者感染了粘质沙雷氏菌，其中188例（73%）表现出ESBL阳性表型。分子分析表明，大多数菌株产生CTX-M和TEMβ-内酰胺酶。在最后一年，产ESBL菌株的百分比下降，但同样在最后一年，我们从三名患者中分离出了对碳青霉烯类耐药的粘质沙雷氏菌。
结论
CTX-M型ESBL在粘质沙雷氏菌菌株产生的ESBL中占主导地位。对碳青霉烯类耐药菌株的出现令人担忧。
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