从移植病房患者中分离出的铜绿假单胞菌对碳青霉烯类抗生素的耐药性。
Resistance to carbapenems among Pseudomonas aeruginosa isolated from patients of transplant wards.
作者信息
Mlynarczyk G, Sawicka-Grzelak A, Szymanek K, Buczkowska T, Pazik J, Durlik M, Pacholczyk M, Lagiewska B, Chmura A, Paczek L, Mlynarczyk A
机构信息
Department of Medical Microbiology, Transplantology and Internal Diseases of Transplantation Institute, The Medical University of Warsaw, Chalubinskiego 5 02-004 Warsaw, Poland.
出版信息
Transplant Proc. 2009 Oct;41(8):3258-60. doi: 10.1016/j.transproceed.2009.08.013.
BACKGROUND
The aim of the study was to estimate the carbapenems resistance and occurrence of metallo-beta-lactamase (MBL) production among Pseudomonas aeruginosa isolated from patients during the last 2 years.
MATERIALS AND METHODS
We investigated all P aeruginosa strains derived from Transplantation Institute patients, hospitalized from January 2007 to December 2008. E-tests as well as the three-discs method with imipenem, ceftazidime were used for MBL detection. For the chosen strains, a PCR method was applied for detection of genes determining VIM, IMP, and SMP.
RESULTS
Among 311 isolated strains from 228 patients only one strain was used for each patient. We showed increased resistance to carbapenems among P aeruginosa in 2008 compared with 2007: from 14% to 22%. About 60% of resistant strains displayed the MBL phenotype. Upon PCR analysis, the VIM-type metallo-beta-lactamase was detected in 70% of them.
CONCLUSIONS
Despite similar numbers of P aeruginosa-infected patients in 2007 and 2008, the percentage of MBL-producing strains increased from 7% to 15%. Most MBLs belonged to the VIM type.
背景
本研究旨在评估过去两年从患者中分离出的铜绿假单胞菌对碳青霉烯类的耐药性以及金属β-内酰胺酶（MBL）的产生情况。
材料与方法
我们调查了2007年1月至2008年12月在移植研究所住院患者的所有铜绿假单胞菌菌株。采用E-test以及亚胺培南、头孢他啶的三纸片法检测MBL。对于所选菌株，应用PCR方法检测决定VIM、IMP和SMP的基因。
结果
从228例患者中分离出311株菌株，每位患者仅使用一株。我们发现，与2007年相比，2008年铜绿假单胞菌对碳青霉烯类的耐药性有所增加：从14%增至22%。约60%的耐药菌株表现出MBL表型。经PCR分析，其中70%检测到VIM型金属β-内酰胺酶。
结论
尽管2007年和2008年铜绿假单胞菌感染患者数量相近，但产生MBL的菌株比例从7%增至15%。大多数MBL属于VIM型。
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