Mereuţă Ana Irina, Tuchiluş Cristina, Bădescu Aida Corina, Iancu Luminiţa Smaranda
Universităţii de Medicină şi Farmacie "Gr. T. Popa" Iaşi.
Rev Med Chir Soc Med Nat Iasi. 2011 Oct-Dec;115(4):1208-13.
The aim of our study was to evaluate the antimicrobial susceptibility profile and the presence of metallo-beta-lactamases (MBLs) among carbapenem-resistant Pseudomonas aeruginosa clinical isolates.
A total of 84 P. aeruginosa clinical isolates collected between January 2007- February 2011 from four university hospitals in Iasi (North-East region of Romania) were randomly selected. Antimicrobial susceptibility testing was performed according to CLSI 2010 (Clinical and Laboratory Standards Institute) guidelines. The isolates were tested for MBLs using EPI (EDTA-phenanthroline-imipenem) phenotypic test and polymerase chain reaction (PCR) for bla(VIM) and bla(IMP).
Fifty-eight carbapenem resistant strains were identified, from which 24 (41,3%) were positive for VIM-type MBLs. No IMP - type MBL was detected. All MBL-producing isolates displayed a MDR (multidrug resistant) phenotype, two of them were XDR (extensively drug-resistant). Colistin remained the most effective antibiotic.
The high proportion of MBL producing P. aeruginosa clinical isolates urges the need for a better use of antibiotics and for efficient infection control measures to prevent dissemination of MBL producers. This is the first report of VIM-like enzymes in P. aeruginosa isolates from the Iasi area.
我们研究的目的是评估耐碳青霉烯类铜绿假单胞菌临床分离株的抗菌药物敏感性谱以及金属β-内酰胺酶（MBLs）的存在情况。
随机选取2007年1月至2011年2月期间从罗马尼亚东北部雅西的四家大学医院收集的84株铜绿假单胞菌临床分离株。根据CLSI 2010（临床和实验室标准协会）指南进行抗菌药物敏感性试验。使用EPI（乙二胺四乙酸-菲咯啉-亚胺培南）表型试验以及针对bla(VIM)和bla(IMP)的聚合酶链反应（PCR）对分离株进行MBLs检测。
鉴定出58株耐碳青霉烯类菌株，其中24株（41.3%）VIM型MBLs呈阳性。未检测到IMP型MBLs。所有产MBLs的分离株均表现出多重耐药（MDR）表型，其中两株为广泛耐药（XDR）。黏菌素仍然是最有效的抗生素。
产MBLs的铜绿假单胞菌临床分离株比例较高促使人们更好地使用抗生素并采取有效的感染控制措施，以防止产MBLs菌株的传播。这是雅西地区铜绿假单胞菌分离株中VIM样酶的首次报道。
