Khosravi Azar D, Mihani Fatemeh
Department of Microbiology, School of Medicine, Ahwaz Jondishapour University of Medical Sciences, Ahwaz, Iran.
Diagn Microbiol Infect Dis. 2008 Jan;60(1):125-8. doi: 10.1016/j.diagmicrobio.2007.08.003. Epub 2007 Sep 27.
The antimicrobial activities of 6 common antimicrobial agents including carbapenems were tested against 100 clinical Pseudomonas aeruginosa isolates using a disk diffusion method. All imipenem (IPM)-resistant isolates were screened for metallo-beta-lactamase (MBL) production by Etest assay and were subsequently subjected to polymerase chain reaction (PCR) analysis with the bla(IMP) and bla(VIM) genes. Of 41 IPM-nonsusceptible isolates detected, 8 (19.51%) appeared to produce MBL, as determined by Etest. Using PCR assay, these isolates were positive for bla(VIM) genes, whereas none were positive for bla(IMP) genes.
采用纸片扩散法，对包括碳青霉烯类在内的6种常见抗菌药物对100株临床分离的铜绿假单胞菌进行抗菌活性测试。通过Etest法对所有耐亚胺培南（IPM）菌株进行金属β-内酰胺酶（MBL）产生情况筛查，并随后用bla(IMP)和bla(VIM)基因进行聚合酶链反应（PCR）分析。在检测出的41株对IPM不敏感的菌株中，经Etest法测定，8株（19.51%）似乎产生MBL。采用PCR检测，这些菌株bla(VIM)基因呈阳性，而bla(IMP)基因均为阴性。
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