The dominant sequence types of vancomycin-resistant Enterococcus faecium among transplantation ward patients.
作者信息
Mlynarczyk A, Szymanek-Majchrzak K, Kosykowska E, Grzybowska W, Tyski S, Mrowka A, Baczkowska T, Durlik M, Pacholczyk M, Chmura A, Ciszek M, Paczek L, Mlynarczyk G
机构信息
Department of Medical Microbiology, Medical University of Warsaw, Poland.
出版信息
Transplant Proc. 2011 Oct;43(8):3132-4. doi: 10.1016/j.transproceed.2011.08.005.
OBJECTIVES
Vancomycin-resistant enterococci (VRE) frequently cause therapeutic problems and provide information about the epidemiological condition of the ward.
MATERIALS AND METHODS
VRE isolated from patients on transplantation wards in 2007-2008 were compared using 2 molecular methods: RFLP-PFGE (restriction fragment length polymorphism-pulse field gel electrophoresis) and MLST (multilocus sequence typing).
RESULTS
The analysis covered 29 Enterococcus faecium strains resistant to glycopeptides, each from a different patient. All organisms were typed using 2 molecular methods. MLST results were compared with an international base. The 30 examined strains belonged to 8 different worldwide known sequence types. All could be recognized as representatives of a single clonal complex CC17.
CONCLUSION
Both methods of typing appeared to be useful to asses the epidemiological condition of the investigated wards.
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