Mlynarczyk A, Szymanek-Majchrzak K, Kosykowska E, Grzybowska W, Tyski S, Mrowka A, Baczkowska T, Durlik M, Pacholczyk M, Chmura A, Ciszek M, Paczek L, Mlynarczyk G
Department of Medical Microbiology, Medical University of Warsaw, Poland.
Transplant Proc. 2011 Oct;43(8):3132-4. doi: 10.1016/j.transproceed.2011.08.005.
Vancomycin-resistant enterococci (VRE) frequently cause therapeutic problems and provide information about the epidemiological condition of the ward.
VRE isolated from patients on transplantation wards in 2007-2008 were compared using 2 molecular methods: RFLP-PFGE (restriction fragment length polymorphism-pulse field gel electrophoresis) and MLST (multilocus sequence typing).
The analysis covered 29 Enterococcus faecium strains resistant to glycopeptides, each from a different patient. All organisms were typed using 2 molecular methods. MLST results were compared with an international base. The 30 examined strains belonged to 8 different worldwide known sequence types. All could be recognized as representatives of a single clonal complex CC17.
Both methods of typing appeared to be useful to asses the epidemiological condition of the investigated wards.
