Domaszewska-Szostek A, Zaleska M, Olszewski W L
Department of Surgical Research & Transplantology, Medical Research Center, Polish Academy of Sciences, 02 106 Warsaw, Poland.
Transplant Proc. 2009 Oct;41(8):3269-71. doi: 10.1016/j.transproceed.2009.07.075.
Cultured keratinocytes (KC) are needed for transplantation to the surface of large burn wounds and ulcers. They can be cultured in artificial media. However, the yield is always limited, viability is low, and proliferation and migration after grafting are slow. The question arose whether tissue fluid/lymph, which is a natural humoral environment for epidermal and dermal cells, contains cytokine(s) specifically regulating KC proliferation and could be used to culture large numbers of cells for transplantation. Culturing of skin keratinocytes in dermal tissue fluid/lymph containing keratinocyte growth factor, interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, and transforming growth factor-beta revealed its strong stimulatory effect on the expression of p63 stem cell marker and proliferation but not differentiation of KC. Neutralizing these cytokines with antibodies resulted in decreased percentages of mitotic figures. None of the individual cytokines showed a dominant effect on proliferation. This observation suggests that either there may be other (so far undetected) specific cytokines or that the proliferation and differentiation of keratinocytes is an effect of the combined action of all investigated cytokines.
培养的角质形成细胞（KC）对于移植到大面积烧伤创面和溃疡表面是必需的。它们可以在人工培养基中培养。然而，产量总是有限的，活力较低，移植后的增殖和迁移也很缓慢。于是产生了一个问题，即作为表皮和真皮细胞天然体液环境的组织液/淋巴中是否含有特异性调节KC增殖的细胞因子，并且能否用于培养大量细胞以供移植。在含有角质形成细胞生长因子、白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α和转化生长因子-β的真皮组织液/淋巴中培养皮肤角质形成细胞，发现其对p63干细胞标志物的表达和KC的增殖有强烈的刺激作用，但对KC的分化没有影响。用抗体中和这些细胞因子会导致有丝分裂相的百分比降低。没有一种单独的细胞因子对增殖表现出主导作用。这一观察结果表明，要么可能存在其他（迄今未检测到的）特异性细胞因子，要么角质形成细胞的增殖和分化是所有研究的细胞因子共同作用的结果。
