Marchese C, Rubin J, Ron D, Faggioni A, Torrisi M R, Messina A, Frati L, Aaronson S A
Institute of General Pathology, University of Catania, Italy.
J Cell Physiol. 1990 Aug;144(2):326-32. doi: 10.1002/jcp.1041440219.
Human keratinocyte growth factor (KGF) is an epithelial cell specific mitogen which is secreted by normal stromal fibroblasts. In the present studies, we demonstrate that KGF is as potent as EGF in stimulating proliferation of primary or secondary human keratinocytes in tissue culture. Exposure of KGF- or EGF-stimulated keratinocytes to 1.0 mM calcium, an inducer of differentiation, led to cessation of cell growth. However, immunologic analysis of early and late markers of terminal differentiation, K1 and filaggrin, respectively, revealed striking differences in keratinocytes propagated in the presence of these growth factors. With KGF, the differentiation response was associated with expression of both markers whereas their appearance was retarded or blocked by EGF. TGF alpha, which also interacts with the EGF receptor, gave a similar response to that observed with EGF. These findings functionally distinguish KGF from the EGF family and support the role of KGF in the normal proliferation and differentiation of human epithelial cells.
人角质形成细胞生长因子（KGF）是一种由正常基质成纤维细胞分泌的上皮细胞特异性促有丝分裂原。在本研究中，我们证明在组织培养中，KGF在刺激原代或传代人角质形成细胞增殖方面与表皮生长因子（EGF）一样有效。将KGF或EGF刺激的角质形成细胞暴露于1.0 mM钙（一种分化诱导剂）会导致细胞生长停止。然而，分别对终末分化的早期和晚期标记物K1和丝聚蛋白进行免疫分析，结果显示在这些生长因子存在的情况下增殖的角质形成细胞存在显著差异。在KGF作用下，分化反应与两种标记物的表达相关，而在EGF作用下它们的出现则延迟或受阻。同样与EGF受体相互作用的转化生长因子α（TGFα）给出了与EGF相似的反应。这些发现从功能上区分了KGF与EGF家族，并支持了KGF在人上皮细胞正常增殖和分化中的作用。
