一种新型保存溶液（SCOT 15）改善了从非心跳供体胰腺中分离胰岛的过程：小鼠模型。

A new preservation solution (SCOT 15) Improves the islet isolation process from pancreata of non-heart-beating donors: a Murine model.

作者信息

Giraud S, Hauet T, Eugene M, Mauco G, Barrou B

机构信息

Institut National de la Santé et de la Recherche Médicale U927, Paris, France.

出版信息

Transplant Proc. 2009 Oct;41(8):3293-5. doi: 10.1016/j.transproceed.2009.08.042.

DOI:10.1016/j.transproceed.2009.08.042

PMID:19857733

Abstract

INTRODUCTION

Due to the organ shortage, there is increased use of organs harvested from non-heart-beating donors (NHBD). These organs have been subjected to a period of warm ischemia that is most deleterious to functional recovery. We have designed a new preservation solution, "Solution de Conservation des Organes et des Tissus" (SCOT 15; Macopharma, Tourcoing, France) which contains an extracellular ionic composition including PEG 20 kD (15 g/L) as a colloid.

METHODS

Our objective was to compare SCOT 15 with University of Wisconsin (UW) solution or islet culture medium CMRL 1066 + 1% of Bovine Serum Albumin (BSA), as the working and preservation solution for islet isolation from pancreata subjected to warm ischemia using a murine model.

RESULTS

Warm ischemia decreased the islet yield and cellular viability regardless of the preservation solution. Either when the pancreas was or was not subjected to warm ischemia, the best islet yield was obtained with SCOT 15 (P < .05 vs UW or CMRL 1066). The same results were observed for islet viability as assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test; namely, better viability with SCOT 15 as compared with UW or CMRL 1066 (P < .01).

CONCLUSION

In a murine model SCOT 15 was a better preservation solution for islet isolation than UW solution or culture medium (CMRL 1066).

摘要

引言

由于器官短缺，来自非心脏跳动供体（NHBD）的器官使用量有所增加。这些器官经历了一段对功能恢复最为有害的热缺血期。我们设计了一种新的保存溶液，即“器官与组织保存溶液”（SCOT 15；法国图尔宽的Macopharma公司），其含有细胞外离子成分，包括作为胶体的聚乙二醇20 kD（15 g/L）。

方法

我们的目的是将SCOT 15与威斯康星大学（UW）溶液或胰岛培养基CMRL 1066 + 1%牛血清白蛋白（BSA）进行比较，作为使用小鼠模型从经历热缺血的胰腺中分离胰岛的工作和保存溶液。

结果

无论使用何种保存溶液，热缺血都会降低胰岛产量和细胞活力。无论胰腺是否经历热缺血，使用SCOT 15时获得的胰岛产量最高（与UW或CMRL 1066相比，P < 0.05）。使用3,（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基溴化四氮唑（MTT）试验评估胰岛活力时也观察到了相同的结果；即，与UW或CMRL 1066相比，SCOT 15的胰岛活力更好（P < 0.01）。