Thorn R M S, Austin A J, Greenman J, Wilkins J P G, Davis P J
Centre for Research in Biomedicine, School of Life Sciences, University of the West of England, Bristol, UK.
J Wound Care. 2009 Aug;18(8):343-6. doi: 10.12968/jowc.2009.18.8.43635.
To compare the antimicrobial effectiveness of silver- and iodine-containing wound dressings against preformed mature biofilms of pathogenic wound bacteria grown in vitro.
Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown within an in vitro flat bed perfusion biofilm model. Mature biofilms were removed and exposed to wound dressings containing either silver or iodine (Aquacel Ag and Iodozyme) within a static diffusion method, for up to 24 hours. This method was designed to reflect certain key features that determine antimicrobial activity within the wound. The numbers of viable bacteria surviving in the biofilms were determined at set time intervals over the test period.
Both test dressings exerted an antimicrobial effect against the target species biofilms, although the iodine dressing was more efficacious under the experimental conditions employed.
There are large and potentially significant differences (as measured in vitro) in the effectiveness of wound dressings containing broad-spectrum antimicrobial agents such as silver and iodine against specific types of bacterial biofilms.
比较含银和含碘伤口敷料对体外培养的致病性伤口细菌预先形成的成熟生物膜的抗菌效果。
在体外平板灌注生物膜模型中培养铜绿假单胞菌和金黄色葡萄球菌的生物膜。去除成熟生物膜，并采用静态扩散法将其暴露于含银或含碘的伤口敷料（爱康银藻酸盐敷料和碘伏酶敷料）中，长达24小时。该方法旨在反映决定伤口内抗菌活性的某些关键特征。在测试期间的设定时间间隔测定生物膜中存活的活菌数量。
两种测试敷料均对目标菌种生物膜产生抗菌作用，尽管在所采用的实验条件下，含碘敷料更有效。
含广谱抗菌剂（如银和碘）的伤口敷料对特定类型细菌生物膜的有效性（体外测定）存在巨大且可能显著的差异。
