Cereceres Stacy, Lan Ziyang, Bryan Laura, Whitely Michael, Wilems Thomas, Greer Hunter, Alexander Ellen Ruth, Taylor Robert J, Bernstein Lawrence, Cohen Noah, Whitfield-Cargile Canaan, Cosgriff-Hernandez Elizabeth
Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843-3120, USA.
Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas 78712, USA.
APL Bioeng. 2019 Apr 29;3(2):026102. doi: 10.1063/1.5088801. eCollection 2019 Jun.
Chronic wounds are projected to reach epidemic proportions worldwide because of the aging population and the increasing incidence of diabetes. Despite extensive research, infection remains one of the leading sources of complications in chronic wounds, resulting in improper healing, biofilm formation, and lower extremity amputation. To address the limitations of standard treatments, we have developed a hydrogel wound dressing with self-tuning moisture control that incorporates a novel antimicrobial agent to eliminate and prevent infection. 3D-printing of a hydrogel dressing with dual porosity resulted in a new dressing with greater flexibility, increased water uptake, and more rapid swelling than bulk hydrogel dressings. Additionally, gallium maltolate (GaM) was incorporated into the dressing to investigate the efficacy of this antimicrobial agent. Loading profiles, release kinetics, and the bactericidal activity against (including methicillin-resistant ) of GaM were investigated to identify target profiles that supported infection control. Finally, GaM-loaded hydrogel dressings were evaluated , utilizing a murine splinted-wound model that was inoculated with . In comparison to an untreated control, GaM dressings markedly reduced the wound bacterial load without compromising wound closure rates. Overall, this work demonstrates the utility of a 3D-printed hydrogel dressing as an antimicrobial dressing to control infection in chronic wounds.
由于人口老龄化和糖尿病发病率的上升，慢性伤口预计将在全球范围内达到流行程度。尽管进行了广泛的研究，但感染仍然是慢性伤口并发症的主要来源之一，导致愈合不当、生物膜形成和下肢截肢。为了解决标准治疗方法的局限性，我们开发了一种具有自调节水分控制功能的水凝胶伤口敷料，该敷料含有一种新型抗菌剂，可消除和预防感染。具有双孔隙率的水凝胶敷料的3D打印产生了一种新的敷料，与块状水凝胶敷料相比，它具有更大的柔韧性、更高的吸水率和更快的膨胀速度。此外，将麦芽酚镓（GaM）加入到敷料中，以研究这种抗菌剂的功效。研究了GaM的负载曲线、释放动力学以及对（包括耐甲氧西林的）的杀菌活性，以确定支持感染控制的目标曲线。最后，利用接种了的小鼠夹板伤口模型对负载GaM的水凝胶敷料进行了评估。与未治疗的对照组相比，GaM敷料显著降低了伤口细菌载量，同时不影响伤口闭合率。总的来说，这项工作证明了3D打印水凝胶敷料作为一种抗菌敷料在控制慢性伤口感染方面的实用性。
