土壤过氧化氢酶活性对铬污染的响应。

Response of soil catalase activity to chromium contamination.

作者信息

Stepniewska Zofia, Wolińska Agnieszka, Ziomek Joanna

机构信息

Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Al. Kraśnicka 102, 20-718 Lublin, Poland.

出版信息

J Environ Sci (China). 2009;21(8):1142-7. doi: 10.1016/s1001-0742(08)62394-3.

DOI:10.1016/s1001-0742(08)62394-3

PMID:19862930

Abstract

The impact of chromium(III) and (VI) forms on soil catalase activity was presented. The Orthic Podzol, Haplic Phaeozem and Mollic Gleysol from different depths were used in the experiment. The soil samples were amended with solution of Cr(III) using CrCl3, and with Cr(VI) using K2Cr2O7 in the concentration range from 0 to 20 mg/kg, whereas the samples without the addition of chromium served as control. Catalase activity was assayed by one of the commonly used spectrophotometric methods. As it was demonstrated in the experiment, both Cr(III) and Cr(VI) have an ability to reduce soil catalase activity. A chromium dosage of 20 mg/kg caused the inhibition of catalase activity and the corresponding contamination levels ranged from 75% to 92% for Cr(III) and 68% to 76% for Cr(VI), with relation to the control. Catalase activity reached maximum in the soil material from surface layers (0-25 cm), typically characterized by the highest content of organic matter creating favorable conditions for microorganisms.

摘要

介绍了铬（III）和铬（VI）形态对土壤过氧化氢酶活性的影响。实验使用了不同深度的灰化土、黑土和潜育土。土壤样品用氯化铬（CrCl₃）溶液添加铬（III），用重铬酸钾（K₂Cr₂O₇）添加铬（VI），浓度范围为0至20毫克/千克，而未添加铬的样品作为对照。过氧化氢酶活性通过常用的分光光度法之一进行测定。实验表明，铬（III）和铬（VI）都有降低土壤过氧化氢酶活性的能力。20毫克/千克的铬剂量导致过氧化氢酶活性受到抑制，相对于对照，铬（III）的相应污染水平为75%至92%，铬（VI）为68%至76%。过氧化氢酶活性在表层（0 - 25厘米）的土壤物质中达到最大值，表层通常以有机质含量最高为特征，为微生物创造了有利条件。