Department of Radiological Life Sciences, Division of Medical Life Sciences, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Japan.
Int J Radiat Biol. 2009;85(10):883-90.
The aim of this study is to evaluate the effects of intermediate frequency (IF) fields generated by induction heating (IH) cooktops from the perspective of cellular genotoxicity and stress responses.
We evaluated the effects of exposure to 23 kHz magnetic fields at 6.05 mT(rms) for 2 h on cellular genotoxicity and stress responses in vitro. The maximum output power in most IH cooktops is at this frequency. The magnetic flux density is approximately 1000 times higher than the reference level in the International Commission on Non-ionising Radiation Protection (ICNIRP) guidelines. For assessment of genotoxicity, we studied cell growth, comet assay, micronucleus formation and hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene mutation. Heat shock protein (Hsp) 27, 70, 105 and phosphorylated Hsp27 were evaluated as indicators of the stress responses.
We did not detect any effects of the IF magnetic fields on cell growth, comet assay, micronucleus formation, HPRT gene mutation, expression of phosphorylated Hsp27, or nuclear translocation of Hsp27, 70 or 105.
Our results indicate that exposure to an IF magnetic field at 6.05 mT(rms) for 2 h does not cause detectable cellular genotoxicity, and does not induce detectable cellular stress.
本研究旨在从细胞遗传毒性和应激反应的角度评估感应加热（IH）炉灶产生的中频（IF）场的影响。
我们评估了在 6.05 mT(rms) 的 23 kHz 磁场中暴露 2 小时对体外细胞遗传毒性和应激反应的影响。大多数 IH 炉灶的最大输出功率在此频率下。磁通密度比国际非电离辐射防护委员会（ICNIRP）指南中的参考水平高约 1000 倍。为了评估遗传毒性，我们研究了细胞生长、彗星试验、微核形成和次黄嘌呤鸟嘌呤磷酸核糖转移酶（HPRT）基因突变。热休克蛋白（Hsp）27、70、105 和磷酸化 Hsp27 被评估为应激反应的指标。
我们没有检测到中频磁场对细胞生长、彗星试验、微核形成、HPRT 基因突变、磷酸化 Hsp27 的表达或 Hsp27、70 或 105 的核转位有任何影响。
我们的结果表明，暴露于 6.05 mT(rms) 的中频磁场 2 小时不会引起可检测的细胞遗传毒性，也不会诱导可检测的细胞应激。
