Remy J, Wegrowski J, Crechet F, Martin M, Daburon F
Laboratoire de Radiobiologie Appliquée, Commissariat à l'Energie Atomique, CEN Saclay, Gif sur Yvette, France.
Radiat Res. 1991 Jan;125(1):14-9.
Collagen metabolism was investigated in the fibrotic tissue which developed in pig thigh muscle 6 to 15 months after acute gamma irradiation. During this period, total collagen deposits in the fibrotic tissue increased 10-fold compared to the healthy muscle tissue. These deposits were composed mainly of type I and III collagen, and the type I/type III ratio was lower in the fibrotic than in the muscle tissue. Small pieces of both fibrotic and muscle tissue were incubated with [14C]proline. The [14C]hydroxyproline content of the fibrotic tissue reflected large concomitant increases in the synthesis of total collagen, mainly of types I and III, which rose 14- and 17-fold, respectively. Similarly, the level of type I and type III procollagen mRNAs rose 9- and 5-fold, respectively, in the fibrotic tissue versus the muscle tissue. These results suggest that procollagen gene transcription or RNA maturation in the cell nuclei is activated in the fibrotic tissue. The possibility that such activation is due to the long-term inflammatory state of this tissue is discussed.
对急性γ射线照射后6至15个月猪大腿肌肉中形成的纤维化组织的胶原代谢进行了研究。在此期间，纤维化组织中的总胶原沉积量与健康肌肉组织相比增加了10倍。这些沉积物主要由I型和III型胶原组成，纤维化组织中I型/III型比例低于肌肉组织。将小块纤维化组织和肌肉组织与[14C]脯氨酸一起孵育。纤维化组织中[14C]羟脯氨酸含量反映出总胶原合成大量增加，主要是I型和III型胶原，分别增加了14倍和17倍。同样，与肌肉组织相比，纤维化组织中I型和III型前胶原mRNA水平分别上升了9倍和5倍。这些结果表明，纤维化组织中细胞核内的前胶原基因转录或RNA成熟被激活。讨论了这种激活可能是由于该组织长期炎症状态的可能性。
