Center for Agricultural Resources Research, Institute of Genetic and Developmental Biology, CAS, Shijiazhuang, 050021, China.
Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan 571339, China.
Int J Mol Sci. 2009 Sep 18;10(9):4116-4136. doi: 10.3390/ijms10094116.
The objective of this study was to locate chromosomes for improving water and phosphorus-deficiency tolerance of wheat at the seedling stage. A set of Chinese Spring-Egyptian Red wheat substitution lines and their parent Chinese Spring (recipient) and Egyptian Red (donor) cultivars were measured to determine the chromosomal locations of genes controlling water use efficiency (WUE) and phosphorus use efficiency (PUE) under different water and phosphorus conditions. The results underlined that chromosomes 1A, 7A, 7B, and 3A showed higher leaf water use efficiency (WUE(l) = Pn/Tr; Pn = photosynthetic rate; Tr = transpiration rate) under W-P (Hoagland solution with 1/2P), -W-P (Hoagland solution with 1/2P and 10% PEG). Chromosomes 7A, 3D, 2B, 3B, and 4B may carry genes for positive effects on individual plant water use efficiency (WUE(p) = biomass/TWC; TWC = total water consumption) under WP (Hoagland solution), W-P and -W-P treatment. Chromosomes 7A and 7D carry genes for PUE enhancement under WP, -WP (Hoagland solution with 10% PEG) and W-P treatment. Chromosome 7A possibly has genes for controlling WUE and PUE simultaneously, which indicates that WUE and PUE may share the same genetic background. Phenotypic and genetic analysis of the investigated traits showed that photosynthetic rate (Pn) and transpiration rate (Tr), Tr and WUE(l) showed significant positive and negative correlations under WP, W-P, -WP and -W-P, W-P, -WP treatments, respectively. Dry mass (DM), WUE(P), PUT (phosphorus uptake) all showed significant positive correlation under WP, W-P and -WP treatment. PUE and phosphorus uptake (PUT = P uptake per plant) showed significant negative correlation under the four treatments. The results might provide useful information for improving WUE and PUE in wheat genetics.
本研究的目的是定位控制小麦幼苗期水分和磷素亏缺耐性的染色体。对一套中国春-埃及红小麦代换系及其亲本中国春（受体）和埃及红（供体）进行了测定，以确定控制水分利用效率（WUE）和磷素利用效率（PUE）的基因在不同水分和磷素条件下的染色体位置。结果表明，染色体 1A、7A、7B 和 3A 在 W-P（Hoagland 溶液含 1/2P）和-W-P（Hoagland 溶液含 1/2P 和 10%PEG）条件下表现出较高的叶片水分利用效率（WUE(l) = Pn/Tr；Pn = 光合速率；Tr = 蒸腾速率）。染色体 7A、3D、2B、3B 和 4B 可能携带正向影响个体水分利用效率（WUE(p) = 生物量/TWC；TWC = 总耗水量）的基因，在 WP（Hoagland 溶液）、W-P 和-W-P 处理下。染色体 7A 和 7D 在 WP、-WP（Hoagland 溶液含 10%PEG）和 W-P 处理下携带增强 PUE 的基因。染色体 7A 可能同时携带控制 WUE 和 PUE 的基因，这表明 WUE 和 PUE 可能具有相同的遗传背景。对所研究性状的表型和遗传分析表明，在 WP、W-P、-WP 和-W-P 处理下，光合速率（Pn）和蒸腾速率（Tr）、Tr 和 WUE(l)之间存在显著的正相关和负相关；在 WP、W-P 和-W-P 处理下，干物质（DM）、WUE(P)和磷素吸收（PUT）之间存在显著的正相关。在四种处理条件下，PUE 和磷素吸收（PUT = 单位植株磷吸收量）呈显著负相关。这些结果可能为提高小麦遗传中 WUE 和 PUE 提供有用信息。
