Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
College of resources, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
BMC Plant Biol. 2020 Jul 13;20(1):330. doi: 10.1186/s12870-020-02492-3.
Phosphorus deficiency is a major limiting factors for affecting crop production globally. To understand the genetic variation of phosphorus-deficiency-tolerance, a total of 15 seedling traits were evaluated among 707 Chinese wheat landraces under application of phosphorus (AP) and non-application of phosphorus (NP). A total of 18,594 single-nucleotide polymorphisms and 38,678 diversity arrays technology sequencing markers were used to detect marker-trait associations under AP and NP.
Top ten genotypes with extremely tolerance and bottommost ten genotypes with extremely sensitivity were selected from 707 Chinese wheat landraces for future breeding and genetic analysis. A total of 55 significant markers (81 marker-trait associations) for 13 traits by both CMLM and SUPER method. These were distributed on chromosomes 1A, 1B, 2A, 2B, 2D, 3A, 4B, 5A, 5B, 6A, 6B, 6D, 7A and 7B. Considering the linkage disequilibrium decay distance, 25 and 12 quantitative trait loci (QTL) were detected under AP and NP, respectively (9 QTL were specific to NP).
The extremely tolerant landraces could be used for breeding phosphorus-deficiency-tolerant cultivars. The QTL could be useful in wheat breeding through marker-assisted selection. Our findings provide new insight into the genetic analysis of P-deficiency-tolerance, and will be helpful for breeding P-deficiency-tolerant cultivars.
磷缺乏是全球影响作物生产的主要限制因素。为了了解磷缺乏耐性的遗传变异，在施加磷（AP）和不施加磷（NP）的情况下，对 707 份中国小麦地方品种的 15 个幼苗性状进行了评估。共检测了 18594 个单核苷酸多态性和 38678 个多样性阵列技术测序标记，以检测 AP 和 NP 下的标记-性状关联。
从 707 份中国小麦地方品种中选择了 10 个具有极强耐性的顶级基因型和 10 个具有极敏感的底层基因型，用于未来的育种和遗传分析。共通过 CMLM 和 SUPER 方法鉴定出 13 个性状的 55 个显著标记（81 个标记-性状关联）。这些标记分布在 1A、1B、2A、2B、2D、3A、4B、5A、5B、6A、6B、6D、7A 和 7B 染色体上。考虑到连锁不平衡衰减距离，在 AP 和 NP 下分别检测到 25 个和 12 个数量性状基因座（QTL）（9 个 QTL 是 NP 特有的）。
这些具有极强耐性的地方品种可用于培育磷缺乏耐性品种。这些 QTL 可通过标记辅助选择在小麦育种中得到利用。我们的研究结果为磷缺乏耐性的遗传分析提供了新的见解，并将有助于培育磷缺乏耐性品种。
