Key Lab of Food Quality and Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
School of Food, Henan Institute of Science and Technology, Xinxiang 453003, China.
Int J Mol Sci. 2009 Sep 23;10(9):4157-4167. doi: 10.3390/ijms10094157.
Boiling water extracts of 66 selected Chinese medicinal herbs were screened for their anticyanobaterial activity against Microcystis aeruginosa by the soft-agar overlayer (SAO) method. Results indicated that extracts from 16 materials could inhibit the growth of this bacterial species. Among these anticyanobacterial samples, eight extracts showed low minimum inhibitory concentrations (MIC), including four extracts with MICs between 1 and 6 mg/mL, and four extracts with MICs < 1 mg/mL which could be considered useful to prevent the outbreak of cyanobacteria before the appearance of cyanobacterial blooms. Further study showed that three extracts with MIC values < 1 mg/mL induced intensive chlorophyll-a lysis within 7 days at the MIC. The results suggested that highly efficient anticyanobacterial compounds must be involved in the inhibitory activities. The final results indicated these three extracts (from Malaphis chinensis, Cynips gallae-tinctoriae and Fructus mume) had the potential to be developed as algicides due to their remarkably anticyanobacterial activities.
采用软琼脂覆盖法，对 66 种中草药的沸水提取物进行了筛选，以检测其对铜绿微囊藻的抗菌活性。结果表明，16 种材料的提取物能够抑制该细菌的生长。在这些抗菌样品中，有 8 种提取物显示出较低的最小抑菌浓度（MIC），其中 4 种提取物的 MIC 在 1-6mg/mL 之间，4 种提取物的 MIC 值<1mg/mL，可以考虑在蓝藻水华出现之前，使用这些提取物来预防蓝藻的爆发。进一步的研究表明，3 种 MIC 值<1mg/mL 的提取物在 MIC 下 7 天内可引起强烈的叶绿素裂解。结果表明，在抑制活性中必然涉及到高效的抗菌化合物。最终结果表明，由于这三种提取物（来自荔枝寄生、杨梅叶蜂和乌梅）具有显著的抗菌活性，因此有可能被开发为杀藻剂。
