School of Science and Health, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia.
Asian Pac J Trop Med. 2013 Sep;6(9):673-81. doi: 10.1016/S1995-7645(13)60117-0.
To evaluate in vitro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.
A total of 58 traditional Chinese medicinal plants were carefully selected based on the literature review and their traditional use. The antimicrobial activities of ethanol extracts of these medicinal plants were tested against fungi (Aspergillus fumigatus), yeast (Candida albicans), gram-negative (Acinetobacter baumannii and Pseudomonas aeruginosa) and gram-positive bacteria (Staphylococcus aureus). The activities were tested at three different concentrations of 1.00, 0.10 and 0.01 mg/mL. The data was analysed using Gene data Screener program.
The measured antimicrobial activities indicated that out of the 58 plant extracts, 15 extracts showed anti-fungal activity and 23 extracts exhibited anti-bacterial activity. Eight plant extracts have exhibited both anti-bacterial and anti-fungal activities. For instance, Eucommia ulmoides, Polygonum cuspidatum, Poria cocos and Uncaria rhyncophylla showed activity against both bacterial and fungal strains, indicating their broad spectrum of activity.
The results revealed that the ethanol extracts of 30 plants out of the selected 58 possess significant antimicrobial activities. It is interesting to note that the findings from the current study are consistent with the traditional use. A clear correlation has also been found between the antimicrobial activity and the flavonoid content of the plant extracts which is in agreement with the literature. Hence, the results presented here can be used to guide the selection of potential plant species for the isolation and structure elucidation of novel antimicrobial compounds in order to establish the structure-activity relationship. This in turn is expected to lead the way to the discovery of novel antimicrobial agents for therapeutic use.
评估 58 种民族药用植物提取物的体外抗菌活性，以期评估其治疗潜力。
根据文献综述和传统用途，精心挑选了总共 58 种传统中药。测试了这些药用植物乙醇提取物对真菌（烟曲霉）、酵母（白色念珠菌）、革兰氏阴性菌（鲍曼不动杆菌和铜绿假单胞菌）和革兰氏阳性菌（金黄色葡萄球菌）的抗菌活性。在 1.00、0.10 和 0.01mg/ml 三个不同浓度下测试了活性。使用 Gene data Screener 程序分析数据。
测量的抗菌活性表明，在 58 种植物提取物中，有 15 种具有抗真菌活性，23 种具有抗细菌活性。有 8 种植物提取物具有抗细菌和抗真菌活性。例如，杜仲、虎杖、茯苓和钩藤对细菌和真菌菌株均有活性，表明其广谱活性。
结果表明，在所选择的 58 种植物中，有 30 种植物的乙醇提取物具有显著的抗菌活性。有趣的是，目前的研究结果与传统用途一致。还发现植物提取物的抗菌活性与类黄酮含量之间存在明显的相关性，这与文献一致。因此，这里提出的结果可用于指导潜在植物物种的选择，以分离和阐明新型抗菌化合物，从而建立结构-活性关系。这反过来有望为治疗用途发现新型抗菌剂铺平道路。
