Bello F, Becerra V
Universidad del Rosario, Bogotá, D.C., Colombia.
Genet Mol Res. 2009 Sep 29;8(3):1179-90. doi: 10.4238/vol8-3gmr652.
In Colombia, the mosquito Ochlerotatus taeniorhynchus has been identified as an efficient vector of the epidemic-epizootic Venezuelan equine encephalitis virus. We evaluated the genetic variability and heterogeneity of this mosquito in Colombian populations using eight microsatellite DNA loci. Two hundred and ten mosquito specimens collected from seven populations of the Colombian Atlantic coast (San Bernardo del Viento, Coveñas, Cartagena, Barranquilla, Ciénaga, Dibulla, and Riohacha) were analyzed. We found five polymorphic microsatellite loci, with 19 alleles giving 62.5% polymorphism; the mean number of alleles per locus was 3.8. The mean expected heterogeneity ranged from 0.568 to 0.660. Most of the polymorphic microsatellite loci were in Hardy-Weinberg disequilibrium, due to both deficit and excess of heterozygotes. The Fst statistic gave a total value of 0.0369, reflecting low genetic differentiation among the populations and, as a consequence, a low degree of structuring among them, while gene flow was high (Nm = 6.52); these findings point to genetic homogeneity among these populations. There was no significant linkage disequilibrium between genotype pairs of the various populations. We concluded that this mosquito is distributed in local populations along the Colombian Atlantic coast; these findings will be useful for developing strategies for controlling this vector.
在哥伦比亚，已确定带纹伊蚊是流行性马脑炎病毒的有效传播媒介。我们使用八个微卫星DNA位点评估了哥伦比亚种群中这种蚊子的遗传变异性和异质性。分析了从哥伦比亚大西洋沿岸七个种群（圣贝尔纳多·德尔比恩托、科夫尼亚斯、卡塔赫纳、巴兰基亚、锡那加、迪布拉和里奥阿查）采集的210只蚊子标本。我们发现了五个多态性微卫星位点，有19个等位基因，多态性为62.5%；每个位点的等位基因平均数为3.8。预期异质性平均值在0.568至0.660之间。大多数多态性微卫星位点处于哈迪-温伯格不平衡状态，这是由于杂合子不足和过剩所致。Fst统计值的总值为0.0369，反映出种群间遗传分化程度低，因此它们之间的结构程度也低，而基因流较高（Nm = 6.52）；这些发现表明这些种群之间存在遗传同质性。不同种群的基因型对之间没有显著的连锁不平衡。我们得出结论，这种蚊子分布在哥伦比亚大西洋沿岸的当地种群中；这些发现将有助于制定控制这种传播媒介的策略。
