Department of Exercise Physiology, Graduate School of Health and Sport Science, Nippon Sport Science University, Tokyo, Japan.
Mol Nutr Food Res. 2010 Mar;54(3):364-72. doi: 10.1002/mnfr.200900145.
We examined whether polyphenols from dietary apple have protective effects against exercise-induced muscle strain injury. Sixteen male Wistar rats were randomly assigned into the apple polyphenol (APP; N=8) and control (CON; N=8) groups. The APP and the CON groups were fed diets with and without 5% APP, respectively. After a 3-wk feeding period, the gastrocnemii of the animals were subject to lengthening contractions with electrical stimulation and forced ankle dorsiflexion. Isometric torques were measured before and after the lengthening contractions and on days 1, 2, 3, 5, and 7 after the contractions. On day 7, the animals were sacrificed and the gastrocnemii harvested. Thiobarbituric-acid-reactive substances, protein carbonyl, and mRNA of antioxidative proteins in the muscles were quantified. The APP group had significantly lower torque deficits than the CON group on days 3, 5, and 7 after the eccentric contractions. The thiobarbituric-acid-reactive substances and protein carbonyl levels in the case of the APP group were significantly lower than those in the case of the CON group. The APP group had significantly higher glutathione-S-transferase alpha1 mRNA levels than the CON group. Therefore, we conclude that dietary APPs have protective effects against lengthening contraction-induced muscle injury.
我们研究了膳食苹果中的多酚是否对运动引起的肌肉劳损损伤具有保护作用。将 16 只雄性 Wistar 大鼠随机分为苹果多酚（APP；N=8）和对照组（CON；N=8）。APP 和 CON 组分别喂食含有和不含有 5%APP 的饮食。经过 3 周的喂养期后，用电刺激和强制踝关节背屈对动物的腓肠肌进行延长收缩。在延长收缩前后以及收缩后第 1、2、3、5 和 7 天测量等长扭矩。第 7 天，处死动物并收获腓肠肌。在肌肉中定量测定了硫代巴比妥酸反应物质、蛋白质羰基和抗氧化蛋白的 mRNA。与对照组相比，APP 组在离心收缩后第 3、5 和 7 天的扭矩缺失明显更低。APP 组的硫代巴比妥酸反应物质和蛋白质羰基水平明显低于对照组。APP 组的谷胱甘肽-S-转移酶 alpha1 mRNA 水平明显高于对照组。因此，我们得出结论，膳食 APP 对延长收缩引起的肌肉损伤具有保护作用。
