Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1922 Mar 31;35(4):553-9. doi: 10.1084/jem.35.4.553.
Cultivation of Bacterium pneumosintes in the collodion sac dialysate of a tissue medium produces an antigen suitable for serological tests. Injection of dialysate cultures of Bacterium pneumosintes into rabbits results in the production of antibodies demonstrable by agglutination, precipitation, complement fixation, and phagocytic reactions. Four strains of Bacterium pneumosintes, three from the first epidemic influenzal wave (1918-19) and one from the second (1920), show identical antigenic characters. The blood serum of rabbits experimentally injected with the glycerolated active material of rabbit passages contains specific agglutinins for Bacterium pneumosintes, whereas normal rabbit serum does not.
在组织培养液胶膜囊中培养肺炎双球菌,可产生适合血清学试验用的抗原。将肺炎双球菌的胶膜囊透析培养物注入兔体,可产生通过凝集、沉淀、补体结合和吞噬反应可证明的抗体。从第一次流感大流行(1918-19)的三个流行株和从第二次流感大流行(1920)的一个流行株中分离的四株肺炎双球菌,显示出相同的抗原特性。用甘油保存的兔传代的活动物质给兔作实验性注射,可使兔血清中含有针对肺炎双球菌的特异凝集素,而正常兔血清则没有。