Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1922 Apr 30;35(5):635-46. doi: 10.1084/jem.35.5.635.
A simple and convenient method is described for the preparation and use of collodion sacs for aerobic and anaerobic bacterial cultivation in vitro. The sacs are suitable for the study of various problems in bacterial growth and metabolism. By their use with the Smith-Noguchi tissue medium, certain microorganisms may be grown in the absence of the confusing, antigenic protein precipitate characteristic of cultures containing fresh tissue, and thus the organisms are obtained in a suitable condition for serological study.
本文介绍了一种简便的方法，用于制备和使用明胶囊，以便在体外进行需氧和厌氧细菌培养。这些明胶囊适用于研究细菌生长和代谢的各种问题。使用 Smith-Noguchi 组织培养基时，某些微生物可以在不含新鲜组织培养物中特征性的抗原性蛋白沉淀的情况下生长，从而使这些生物体在适合血清学研究的条件下获得。
