Yeh H J, Ruit K G, Wang Y X, Parks W C, Snider W D, Deuel T F
Department of Medicine, Washington University Medical Center, Jewish Hospital, St. Louis, Missouri 63110.
Cell. 1991 Jan 11;64(1):209-16. doi: 10.1016/0092-8674(91)90222-k.
Platelet-derived growth factor (PDGF) may be a critical factor in the temporal differentiation of glial elements in the mammalian central nervous system. We have used in situ hybridization and immunoperoxidase staining to investigate the localization of PDGF A and have observed high levels of PDGF A-chain mRNA and immunoreactive PDGF A in neurons of embryonic and adult mice. PDGF A-chain expression was shown to be developmentally regulated and tissue specific. Every neuronal population examined in the central and peripheral nervous systems expresses PDGF A transcripts. Variable, significantly weaker signals are observed in glial cells. In contrast to known neurotrophic factors, the PDGF A transcripts are widely distributed among neurons. This generalized distribution of PDGF A transcripts, together with the known effects of PDGF on glial cells in vitro, suggests a unique role of neurons in regulating the proliferation and differentiation of glial cells in vivo.
血小板衍生生长因子（PDGF）可能是哺乳动物中枢神经系统中神经胶质细胞时序分化的关键因素。我们运用原位杂交和免疫过氧化物酶染色法来研究PDGF A的定位，并在胚胎期和成年期小鼠的神经元中观察到高水平的PDGF A链mRNA和免疫反应性PDGF A。结果显示，PDGF A链的表达受发育调控且具有组织特异性。在中枢神经系统和周围神经系统中检测的每个神经元群体均表达PDGF A转录本。在神经胶质细胞中观察到的信号变化较大且明显较弱。与已知的神经营养因子不同，PDGF A转录本广泛分布于神经元中。PDGF A转录本的这种广泛分布，连同PDGF在体外对神经胶质细胞的已知作用，提示神经元在体内调节神经胶质细胞增殖和分化中具有独特作用。
