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旋毛形螺旋体:关于其生活史和培养的说明。

SPIROCHAETA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION.

机构信息

Department of Pathology and Bacteriology of the Johns Hopkins University, Baltimore.

出版信息

J Exp Med. 1922 Nov 30;36(6):617-26. doi: 10.1084/jem.36.6.617.

Abstract
  1. Pure lines of Spirochoeta eurygyrata were isolated and cultured. 2. They lived longest in a medium which was made up of sodium chloride and pig serum water. 3. They stained readily with iron-hematoxylin, Giemsa's stain, Cross' stain, and carbolfuchsin. 4. They did not stain with the vital dyes used. 5. They divide by transverse division. 6. Transverse bars, which are seen in stained spirochetes and in dead ones with dark-field illumination, are considered coagulated protoplasm, due to death. 7. Coccoid bodies were not seen in living individuals nor were they seen free in the culture medium. 8. Cats were fed cultures of Spirochoeta eurygyrata but did not become infected with them. 9. Spirochoeta eurygyrata is not of frequent occurrence in this part of the United States.
摘要
  1. 分离并培养了旋毛虫的纯系。

  2. 它们在由氯化钠和猪血清水组成的培养基中生存时间最长。

  3. 它们易于用铁苏木精、吉姆萨染色、克罗斯染色和卡宝复红染色。

  4. 它们不会被使用的活体染料染色。

  5. 它们通过横向分裂进行繁殖。

  6. 在染色的旋毛虫和暗场照明下的死旋毛虫中可以看到横向条带,这些条带被认为是由于死亡而凝固的原生质。

  7. 在活体内未观察到球菌体,也未在培养基中观察到游离的球菌体。

  8. 给猫喂食旋毛虫培养物,但它们没有被感染。

  9. 在美国的这一地区,旋毛虫并不常见。

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