Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1925 Aug 31;42(3):419-29. doi: 10.1084/jem.42.3.419.
When bacteriophage is precipitated by alcohol at room temperature its activity rapidly and progressively decreases until it is totally destroyed, between 6 and 24 hours after exposure. If the percipitation is carried out at 7 degrees C. the destruction of lytic activity is considerably slower; measurable traces may be detected even after 4 weeks exposure to alcohol. Although the major portion of the lytic activity is found in the precipitate, the supernatant alcohol carries a measurable amount of lytic principle which remains active for several days. In all cases the residual lytic activity was found to be transmissible in series. In no instance were we able to observe the non-transmissible action ascribed by d'sHérelle to the enzyme. The persistence of traces of active principle after many weeks of exposure to alcohol at low temperature is not found to be due to the existence in the original filtrate of a fraction relatively resistant to the effect of alcohol. The inactivation of bacteriophage by alcohol seems, therefore, analogous to the alcoholic inactivation of certain enzymes and toxins.
当噬菌体在室温下用酒精沉淀时，其活性会迅速且逐渐下降，在暴露后 6 至 24 小时内完全失活。如果在 7°C 下进行沉淀，溶菌活性的破坏速度会明显减慢；即使在暴露于酒精 4 周后，仍能检测到可测量的痕迹。尽管大部分溶菌活性存在于沉淀物中，但上清液中的酒精仍携带可测量数量的溶菌因子，这种因子在数天内保持活性。在所有情况下，残留的溶菌活性都被发现具有可传递性。在任何情况下，我们都未能观察到 d 'Hérelle 归因于酶的不可传递作用。在低温下用酒精暴露数周后，仍能检测到活性物质的痕迹，这并不是因为原始滤液中存在对酒精作用相对有抵抗力的部分。酒精对噬菌体的失活作用似乎类似于某些酶和毒素的酒精失活作用。
