Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1925 Nov 30;42(6):821-8. doi: 10.1084/jem.42.6.821.
Lytic filtrates, active against Bacillus dysenterioe Shiga, Bacillus coli, Bacillus pestis cavioe, and staphylococcus respectively, proved to be differently affected by changes in hydrogen ion concentration. Anti-staphylococcus lysin was the least resistant of the four, showing deterioration in 3 hours at 7 degrees C. beyond the zone of hydrogen ion concentration limited by C(H) = 6.3 x 10(-5) and C(H) = 1.6 x 10(-9). Under the same conditions, the zone of resistance of anti-coli filtrate lay between C(H) = 2.7 x 10(-3) and C(H) = 2.5 x 10(-11), and that of anti-Shiga between C(H) = 1-7 x 10(-4) and C(H) = 1-3 x 10(-11). Anti-pestis cavioe filtrate was most resistant of the four, retaining its full activity in the zone from C(H) = 1 x 10(-3) to C(H) = 3.5 x 10(-12). The fact that these differences in individual resistance persisted, notwithstanding the repeated passage of lytic filtrates through cultures of bacteria other than those against which they were primarily active, seems to offer evidence in favor of a multiplicity of bacteriophages.
溶菌滤液分别对痢疾杆菌、大肠杆菌、鼠疫巴斯德氏菌和金黄色葡萄球菌具有活性，结果表明其对氢离子浓度变化的影响各不相同。抗金黄色葡萄球菌溶菌素是这四种中最不耐受的，在 7°C 下，氢离子浓度的变化范围为 C(H) = 6.3 x 10(-5) 至 C(H) = 1.6 x 10(-9)时，其性能在 3 小时内恶化。在相同条件下，抗大肠杆菌滤液的抗性范围在 C(H) = 2.7 x 10(-3) 至 C(H) = 2.5 x 10(-11)之间，抗志贺氏菌滤液的抗性范围在 C(H) = 1-7 x 10(-4) 至 C(H) = 1-3 x 10(-11)之间。抗鼠疫巴斯德氏菌滤液是这四种中最耐受力最强的，在氢离子浓度范围从 C(H) = 1 x 10(-3) 至 C(H) = 3.5 x 10(-12)时，其仍保持全部活性。尽管溶菌滤液反复通过非主要活性的细菌培养物，但个体抗性的这些差异仍然存在，这似乎为多种噬菌体提供了证据。
