Department of Bacteriology, New York University and Bellevue Medical College, New York.
J Exp Med. 1928 Jan 1;47(1):57-92. doi: 10.1084/jem.47.1.57.
The mechanism of the precipitin reaction between antipneumococcus sera and the type-specific soluble carbohydrate is investigated. The sensitivity of the reaction is found to be generally constant when expressed by the product of the concentrations of the two reacting substances. Precipitin index (P.I.) is defined as one-millionth of the reciprocal value of this product. See PDF for Structure Zonal phenomena and their bearing on the absolute concentration and the equivalent weight of the antibody are discussed. The greater tendency towards exhibition of a post-zone in Type III is connected with the lower acid equivalent of the homologous specific carbohydrate. The influence of the addition of normal serum and the influence of slight changes in pH are studied. The addition of normal serum as well as the increase in pH promote the pro-zone, whereas decrease in pH promotes the post-zone. The sensitivity of the reaction is accordingly influenced by the pH in different ways depending on the range of concentration. The precipitin index allows the recognition and elimination of zonal irregularities. Thus it offers a method for the standardization of pneumonia antibody.
研究了抗肺炎球菌血清与特异可溶性碳水化合物之间沉淀反应的机制。发现当用两个反应物浓度的乘积来表示时，反应的灵敏度通常是恒定的。沉淀指数 (P.I.) 定义为该乘积倒数的百万分之一。请参阅 PDF 以了解结构区域现象及其与抗体的绝对浓度和当量的关系。III 型中后区表现出更大的趋势与同系特异碳水化合物的较低酸当量有关。研究了正常血清的添加和 pH 值稍有变化的影响。添加正常血清和增加 pH 值都会促进前区，而降低 pH 值则会促进后区。因此，反应的灵敏度会根据浓度范围以不同的方式受到 pH 值的影响。沉淀指数允许识别和消除区域不规则性。因此，它为肺炎抗体的标准化提供了一种方法。
