Hospital of The Rockefeller Institute for Medical Research.
J Exp Med. 1928 Nov 30;48(6):769-90. doi: 10.1084/jem.48.6.769.
The matt and the glossy forms of four strains of hemolytic streptococci were used to immunize rabbits. Precipitin tests showed that rabbit sera prepared against matt organisms, whether virulent or avirulent for mice, contained type-specific antibody while sera prepared against completely degraded glossy organisms contained no type-specific antibody. Type-specific antibody was removed from the sera by absorption with homologous matt organisms but was unaffected by absorption with homologous glossy organisms. Passive protection experiments on mice showed that anti-matt sera were protective and anti-glossy sera non-protective against infection with homologous virulent organisms. Vaccination of mice with matt organisms rendered them immune to subsequent infection with homologous virulent cultures; but vaccination with glossy organisms established no active immunity.
四种溶血性链球菌的粗糙型和光滑型分别免疫家兔。沉淀试验表明，家兔针对粗糙型、不论是对小白鼠有毒力的或无毒力的生物体所制备的血清，含有型特异性抗体，而针对完全降解的光滑型生物体所制备的血清则不含有型特异性抗体。用同种粗糙型生物体吸收可以除去血清中的型特异性抗体，但用同种光滑型生物体吸收则不影响型特异性抗体。用粗糙型生物体对小白鼠进行被动保护实验表明，抗粗糙型血清对同源有毒力生物体的感染具有保护作用，而抗光滑型血清则无保护作用。用粗糙型生物体对小白鼠进行免疫接种可使它们对随后的同源有毒力培养物的感染产生免疫力；但是用光滑型生物体进行接种则不能建立主动免疫力。
