用亚甲基蓝耐受性和最终酸度区分人源和乳源溶血性链球菌。
DIFFERENTIATION OF HEMOLYTIC STREPTOCOCCI OF HUMAN AND OF DAIRY ORIGIN BY METHYLENE BLUE TOLERANCE AND FINAL ACIDITY.
机构信息
Department of Bacteriology and Immunology, School of Medicine, Vanderbilt University, Nashville, Tennessee.
出版信息
J Exp Med. 1929 Sep 30;50(4):463-9. doi: 10.1084/jem.50.4.463.
A grouping of 138 strains of hemolytic streptococci based on differences in dye-sensitiveness and in final hydrogen-ion concentration of cultures is presented. Three groups are distinguished; (1) human parasitic strains, defined by a final pH range of 5.2 to 5.0 and by failure to reduce methylene blue (1:5000) in milk, (2) bovine strains parasitic in the udder, characterized by a final pH range of 4.5 to 4.2 and by failure to reduce methylene blue (1:5000) in milk, (3) saprophytic strains, characterized by a final pH range of 4.5 to 4.2 and by ability to reduce methylene blue. Methylene blue was bactericidal for the strains of hemolytic streptococci that fail to reduce it, but neither bacteriostatic nor bactericidal for the strains that caused its reduction.
本文介绍了根据染料敏感性和培养物最终氢离子浓度的差异，对 138 株溶血性链球菌进行分组的情况。区分出三个组：（1）人寄生菌株，其最终 pH 值范围为 5.2 至 5.0，并且不能还原牛奶中的亚甲蓝（1:5000），（2）乳房寄生的牛菌株，其最终 pH 值范围为 4.5 至 4.2，并且不能还原牛奶中的亚甲蓝（1:5000），（3）腐生菌株，其最终 pH 值范围为 4.5 至 4.2，并且能够还原亚甲蓝。对于不能还原亚甲蓝的溶血性链球菌菌株，亚甲蓝具有杀菌作用，但对导致其还原的菌株既没有抑菌作用也没有杀菌作用。
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