Facklam R R, Moody M D
Appl Microbiol. 1970 Aug;20(2):245-50. doi: 10.1128/am.20.2.245-250.1970.
Six tests commonly used for the presumptive identification of group D streptococci were evaluated. Strains tested included 282 group D streptococci and 366 non-group D. Ratios of percentages of group D to non-group D strains which gave positive reactions for each test are as follows: bile-esculin, 100:2; salt tolerance, 88:24; heat tolerance, 100:80; SF broth, 86:1; KF broth, 99:40; and methylene blue milk reduction, 90:17. These data indicate that the bile-esculin test provided a reliable means of identifying group D streptococci and differentiating them from non-group D streptococci. Methodology for reading and interpreting positive reactions and time of incubation of the bile-esculin medium was defined. Evidence of the need for standardization of salt and heat-tolerance tests was obtained.
对常用于初步鉴定D群链球菌的六项试验进行了评估。所检测的菌株包括282株D群链球菌和366株非D群链球菌。每种试验中D群与非D群菌株呈阳性反应的百分比比值如下：胆汁七叶苷试验，100:2；耐盐试验，88:24；耐热试验，100:80；SF肉汤试验，86:1；KF肉汤试验，99:40；亚甲蓝牛奶还原试验，90:17。这些数据表明，胆汁七叶苷试验为鉴定D群链球菌并将其与非D群链球菌区分开来提供了一种可靠的方法。确定了胆汁七叶苷培养基阳性反应的读取和解释方法以及孵育时间。获得了盐和耐热试验标准化必要性的证据。
