从β 溶血性链球菌中分离得到一种稳定的溶血素-白细胞毒素及其结晶衍生物。
A STABLE HEMOLYSIN-LEUCOCIDIN AND ITS CRYSTAL-LINE DERIVATIVE ISOLATED FROM BETA HEMOLYTIC STREPTOCOCCI.
机构信息
Department of Bacteriology, School of Medicine, University of Pennsylvania, Philadelphia.
出版信息
J Exp Med. 1938 Mar 31;67(4):643-57. doi: 10.1084/jem.67.4.643.
Abstract
- A chemically pure hemolysin-leucocidin has been isolated from beta hemolytic streptococci, but not from other species of bacteria studied. 2. It does not give rise to antibodies, but precipitates immune sera against hemolytic streptococci, and is therefore a hapten. 3. A highly purified sample of S. H. up to a dilution of 1:128,000 hemolyzes red blood cells. Its hemolytic activity is not specifically neutralized by antiserum versus beta hemolytic streptococci. It is leucocidic in that it inhibits the reduction of methylene blue by leucocytes. 4. The hemolysin-leucocidin is stable to oxygen, to heat and to moderate changes in hydrogen ion concentration. Its chemical structure has been determined in part. Its molecular weight is 2260. 5. A crystalline derivative has been isolated as the sodium salt from the hemolysin-leucocidin. As the free acid it has a molecular weight of 720. Its hemolytic and leucocidic activity parallels that of S. H., although it is not serologically active. It possesses a high degree of toxicity for mice and rabbits.
摘要
一种化学纯的溶菌素-白细胞毒素已从β溶血性链球菌中分离出来，但从研究的其他细菌种中未分离出来。
它不会引起抗体，但可沉淀针对溶血性链球菌的免疫血清，因此是半抗原。
高度纯化的 S.H.样品可稀释至 1:128000 使红细胞溶血。其溶血活性不能被针对β溶血性链球菌的抗血清特异性中和。它具有白细胞毒素活性，可抑制白细胞对亚甲蓝的还原。
溶菌素-白细胞毒素对氧、热和氢离子浓度的适度变化稳定。其化学结构已部分确定。分子量为 2260。
已从溶菌素-白细胞毒素中分离出一种作为钠盐的结晶衍生物。作为游离酸，其分子量为 720。其溶血和白细胞毒素活性与 S.H.平行，尽管它在血清学上没有活性。它对小鼠和兔子具有高度毒性。
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2
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3
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