Laboratories of the Mount Sinai Hospital, New York.
J Exp Med. 1931 Jun 30;54(1):1-10. doi: 10.1084/jem.54.1.1.
It has proved possible to elicit passive immunity to B. typhosus reacting factors by means of normal and immune homologous neutralizing antibodies. The in vivo serum protection against these factors followed the law of multiple proportions. There was observed a considerable loss of antibodies from the blood stream. Passive immunity was best obtained when the immune serum was injected intravenously (1/2) hour before the intravenous injection of the reacting factors. It was possible to prevent the occurrence of the local skin reaction by an intravenous injection of serum after the intravenous injection of the reacting factors, provided the serum dose was very large and provided the serum injection was made immediately after the filtrate injection. A number of experiments clearly demonstrated the interesting fact that the greater the amount of antiserum injected intravenously, the more efficient was the in vivo neutralization, in a ratio distinctly greater than the quantitative increase of serum. It is suggested that there may be a practical value of the observation in relation to serum therapy. The results also demonstrated passive serum protection against the lethal effect of B. typhosus "agar washings" filtrates, in a ratio which seemed to suggest the law of multiple proportions.
已经证明，通过正常和免疫同源中和抗体，可以引起对 B. typhosus 反应因子的被动免疫。体内对这些因子的血清保护作用遵循多重比例定律。从血流中观察到抗体的大量损失。当免疫血清在反应因子静脉注射前 1/2 小时静脉内注射时，被动免疫效果最佳。在反应因子静脉注射后，通过静脉内注射血清可以防止局部皮肤反应的发生，只要血清剂量非常大，并且在过滤液注射后立即进行血清注射即可。许多实验清楚地表明了一个有趣的事实，即静脉内注射的抗血清量越大，体内中和的效率就越高，比例明显大于血清的定量增加。这表明观察结果可能与血清疗法有实际价值。结果还表明，被动血清对 B. typhosus“琼脂洗出液”滤液的致死作用具有保护作用，其比例似乎表明多重比例定律。
