Laboratories of The Mount Sinai Hospital, New York.
J Exp Med. 1935 Feb 28;61(3):383-93. doi: 10.1084/jem.61.3.383.
In this paper there are described methods for grading the local skin reactivity induced by bacterial filtrates. The grading is accomplished by titration of the skin-preparatory factors; and by studies on the duration of ensuing reactivity. It was found that the duration of reactivity depends upon the mode of preparation of filtrates and the microorganisms employed. Thus, it lasts for 96 hours with meningococcus "agar washings" filtrates; for 72 hours with B. typhosus "agar washings" filtrates; it disappears within 48 hours with B. typhosus tryptic digest broth culture filtrate and B. typhosus "agar washings" filtrates previously heated in the Arnold sterilizer for 20 minutes. Comparative titrations of the preparations employed demonstrate that the duration of reactivity is in direct relationship to the reacting potency. It is also shown that the skin-preparatory potency of the filtrates and the duration of the ensuing local reactivity are not modified by cellular anaphylactic sensitization (Arthus phenomenon) to animal proteins. The exposure of tissues to the effect of certain soluble bacterial factors induces a high susceptibility to humoral toxic principles resulting from intravascular antigen-antibody interaction.
本文介绍了用于分级细菌滤液引起的局部皮肤反应的方法。通过滴定皮肤预备因素和研究随后反应的持续时间来完成分级。结果发现，反应的持续时间取决于滤液的制备方式和所用的微生物。因此，脑膜炎球菌“琼脂洗液”滤液的反应持续时间为 96 小时；伤寒杆菌“琼脂洗液”滤液的反应持续时间为 72 小时；伤寒杆菌胰蛋白酶消化肉汤培养液滤液和先前在阿诺德灭菌器中加热 20 分钟的伤寒杆菌“琼脂洗液”滤液的反应持续时间在 48 小时内消失。对所使用的制剂的比较滴定表明，反应持续时间与反应效力直接相关。还表明，滤液的皮肤预备效力和随后的局部反应持续时间不受对动物蛋白的细胞过敏致敏（阿瑟斯现象）的影响。组织暴露于某些可溶性细菌因子的作用会导致对血管内抗原抗体相互作用产生的体液毒性原理产生高度敏感性。
