斑疹伤寒：二、感染斑疹伤寒豚鼠阴囊渗出物的细胞学研究。

TYPHUS FEVER : II. CYTOLOGICAL STUDIES OF THE SCROTAL SAC EXUDATE IN TYPHUS-INFECTED GUINEA PIGS.

Department of Pathology, Harvard Medical School, Boston.

J Exp Med. 1931 Jul 31;54(2):187-95. doi: 10.1084/jem.54.2.187.

Abstract

A satisfactory method is described for the topographical study of Rickettsia prowazeki in sections of the scrotal sac of typhus-infected guinea pigs. 2. In such sections Rickettsiae are always intracellular. 3. Typhus Rickettsiae multiply luxuriantly in the serosal cells and produce great distention of these cells. 4. Rickettsiae may be found in small numbers in the endothelial cells lining the underlying capillaries in the testes and scrotum, but are not seen in perivascular macrophages, connective tissue cells, fat cells, smooth or striated muscle fibres or epithelial cells. 5. Rickettsiae are rarely phagocytosed in small numbers by polymorphonuclears but are never seen in lymphocytes, plasma cells or eosinophiles. 6. When infected mesothelial cells proliferate and desquamate, they rapidly lose their content of Rickettsiae. 7. The large mononuclear cells seen in smears of the scrotal sac exudate may be separated into two groups: (1) the serosal (mesothelial) cells which become heavily infected with Rickettsiae but which are not phagocytic for graphite ink and (2) the macrophages (phagocytic cells) which do not contain Rickettsiae.

摘要

描述了一种满意的方法，用于对感染斑疹伤寒的豚鼠阴囊切片中的普氏立克次体进行局部研究。
在这些切片中，立克次体总是存在于细胞内。
斑疹伤寒立克次体在浆膜细胞中大量繁殖，并使这些细胞极度扩张。
在睾丸和阴囊的基底毛细血管内皮细胞中可以发现少量的立克次体，但在血管周围巨噬细胞、结缔组织细胞、脂肪细胞、平滑肌或横纹肌纤维或上皮细胞中则看不到。
少数多形核白细胞偶尔会吞噬少量的立克次体，但在淋巴细胞、浆细胞或嗜酸性粒细胞中则看不到。
当受感染的间皮细胞增殖和脱落时，它们很快就失去了立克次体的内容物。
在阴囊渗出物的涂片上可见到的大型单核细胞可分为两类：（1）浆膜（间皮）细胞，它们受到立克次体的严重感染，但不吞噬石墨墨水；（2）巨噬细胞（吞噬细胞），其中不含有立克次体。