Department of Pathology, Harvard Medical School, Boston.
J Exp Med. 1931 Jul 31;54(3):307-14. doi: 10.1084/jem.54.3.307.
Typhus Rickettsiae are found in large numbers in sections of tissue cultures of scrotal sac exudate. Extensive multiplication of the organisms occurs, and new cells become infected. Organisms are seen in cells undergoing mitotic division. The organisms usually become less numerous after the 6th day in vitro, but in one instance organisms were extremely numerous on the 16th and 21st days. Rickettsiae in tissue cultures retain their intracellular location, even when infection is very heavy. They multiply exclusively in non-phagocytic cells which are believed to be of mesothelial origin. Pleomorphism is much more pronounced in tissue cultures than in guinea pig tissues and is entirely comparable to that seen in the louse.
恙虫病立克次体在阴囊渗出物组织培养物的某些部位大量存在。生物体会大量繁殖，新的细胞会受到感染。可以在有丝分裂的细胞中看到生物。在体外第 6 天后，生物通常会减少，但在一个实例中，第 16 天和第 21 天的生物数量非常多。组织培养物中的立克次体即使感染非常严重，仍保持其细胞内位置。它们仅在非吞噬细胞中繁殖，这些细胞被认为来自间皮细胞。在组织培养物中，多形性比豚鼠组织中更为明显，与虱子中看到的完全可比。
