暴露于超正常温度下的组织培养的生长和死亡。
THE GROWTH AND DEATH OF TISSUE CULTURES EXPOSED TO SUPRANORMAL TEMPERATURES.
机构信息
Laboratory of General Physiology, Harvard University, Cambridge, Massachusetts, and the Guest Laboratory of A. Fischer, Kaiser Wilhelm Institute for Biology, Berlin-Dahlem, Germany.
出版信息
J Exp Med. 1931 Jul 31;54(3):323-32. doi: 10.1084/jem.54.3.323.
In a study of the growth of chicken osteoblasts in cultures exposed to supranormal temperatures of 42 degrees C., 44 degrees C., 47 degrees C., 50 degrees C., and 52 degrees C., it has been observed that: 1. There is no lethal effect after prolonged exposures at 42 degrees C. and 44 degrees C. 2. Cultures are killed after an exposure of 105 minutes at 47 degrees C., of 6 minutes at 50 degrees C., and of 3.5 minutes at 52 degrees C. 3. A definite inhibition of growth occurs after different exposures at all temperatures from 44 degrees C. onwards (see Table I). 4. There is a latent period of approximately 24 hours before any discernible effect of sublethal or just lethal exposures; this latent period appears to be independent of the duration of the sublethal exposure and of the temperature. 5. The high temperature coefficients for lethal exposures and for exposures just sufficient to inhibit growth indicate an underlying "destructive" process in the cells of the culture.
在一项研究中，将鸡成骨细胞置于温度分别为 42°C、44°C、47°C、50°C 和 52°C 的超正常温度下培养，观察到：1. 在 42°C 和 44°C 下长时间暴露，没有致死效应。2. 47°C 下暴露 105 分钟，50°C 下暴露 6 分钟，52°C 下暴露 3.5 分钟，细胞就会死亡。3. 所有温度（见附表 I）从 44°C 起，细胞生长受到不同程度的抑制。4. 在亚致死或仅致死暴露后，需要大约 24 小时才会出现明显的效果；这个潜伏期似乎与亚致死暴露的持续时间和温度无关。5. 致死暴露和足以抑制生长的暴露的高温系数表明培养细胞中存在潜在的“破坏性”过程。
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