Department of Pathology, The Harvard Medical School, Boston.
J Exp Med. 1936 Aug 31;64(3):485-502. doi: 10.1084/jem.64.3.485.
Various types of inflammatory exudates have been obtained either by the introduction into normal tissues of a chemical irritant, or by a burn, or by bacteria in either dogs or rabbits. A study has also been made on an exudate of human origin. These exudates have all been found to contain a factor which induces prompt increase in the permeability of normal skin capillaries, demonstrable by the almost immediate accumulation from the circulation of trypan blue into areas of skin injected with the cell-free exudate. The active factor may be carried down with the precipitate resulting from the interaction of the exudate with either saturated ammonium sulfate or 20 per cent sodium sulfate. The active factor passes through a dialyzing membrane. It can be recovered from the dialysate as a protein-free crystalline material. The active factor manifests no property in common with histamine or presumably with the hypothetical H substance assumed to be closed related to histamine. This is indicated by the following considerations: (a) difference between the tissue staining pattern of the exudate or of its active fraction and that of histamine; (b) opposite effects by histamine and the active factor found in exudates on the tonicity of the isolated strip of guinea pig intestine. The observations presented in this report do not substantiate Lewis' hypothesis of histamine or of its closely related H substance as the primary cause of increased capillary permeability in inflammation. The present studies are being continued in an endeavor to free of its impurities and to identify the active crystalline-like material isolated from an inflammatory exudate. The details of this investigation will form the subject of a separate future communication.
已通过向正常组织中引入化学刺激物、烧伤或狗或兔中的细菌获得各种类型的炎性渗出物。还对源自人体的渗出物进行了研究。所有这些渗出物都被发现含有一种因子，该因子可立即增加正常皮肤毛细血管的通透性，可通过将锥虫蓝从循环中迅速积聚到用无细胞渗出物注射的皮肤区域来证明。活性因子可随渗出物与饱和硫酸铵或 20％硫酸钠相互作用而产生的沉淀物一起下降。活性因子可通过透析膜。可以从透析液中回收无蛋白的结晶物质作为活性因子。活性因子与组胺或假设的与组胺密切相关的 H 物质没有共同特性。以下考虑表明了这一点：（a）渗出物或其活性部分与组胺的组织染色模式之间的差异；（b）组胺和渗出物中发现的活性因子对豚鼠肠分离带的渗透压的相反作用。本报告中提出的观察结果并不支持刘易斯关于组胺或其密切相关的 H 物质作为炎症中毛细血管通透性增加的主要原因的假说。目前正在继续进行这些研究，以清除其杂质并鉴定从炎性渗出物中分离出的活性类似晶体物质。这项研究的细节将成为未来单独通讯的主题。
