Department of Medicine, Western Reserve University, School of Medicine, Cleveland.
J Exp Med. 1938 Apr 30;67(5):725-38. doi: 10.1084/jem.67.5.725.
A method has been described by which it is possible to estimate the number of particles of vaccine virus which are required to cause infection in the rabbit skin. The method consists essentially in performing a series of intradermal inoculations in rabbits of suitably diluted virus suspensions. The percentage of inoculations at each dilution giving rise to lesions is observed, and the data are subjected to appropriate statistical analysis. Several strains of vaccine virus, differing in their characteristics, have been studied with the following results. Infection with the New York City Board of Health virus appears to follow the injection of a single particle of virus. The same is true for the strain derived from it but cultured in a chick embryo-Tyrode solution medium for a prolonged period. This strain, as has been noted, has largely lost its ability to cause extensive necrosis in the rabbit skin, and causes generalized infection only exceptionally. From the results here reported, it appears that other factors are responsible for the altered character of the lesion than the ability of the virus to establish a foothold in the animal organism. In this respect the cultured appears to be the equal of the original passage virus. Similarly the Noguchi strain of virus is apparently capable of infecting, if a single virus particle is properly introduced.
已经描述了一种方法，可以估计引起兔皮感染所需的疫苗病毒粒子的数量。该方法主要包括在兔皮中进行一系列适当稀释的病毒悬浮液的皮内接种。观察每种稀释度导致病变的接种百分比，并对数据进行适当的统计分析。已经用以下结果研究了几种特征不同的疫苗病毒。用纽约市卫生局病毒进行的感染似乎遵循单个病毒粒子的注射。从它衍生而来但在鸡胚-泰罗溶液培养基中长时间培养的菌株也是如此。正如已经指出的，这种菌株已经大大丧失了在兔皮中引起广泛坏死的能力，并且仅异常地引起全身性感染。从这里报道的结果来看，似乎除了病毒在动物机体中立足的能力之外，还有其他因素导致病变特征的改变。在这方面，培养物似乎与原始传代病毒一样。同样，诺古奇病毒株显然能够感染，如果适当引入单个病毒粒子的话。
