Laboratories of the International Health Division, The Rockefeller Foundation, New York.
J Exp Med. 1938 May 31;67(6):953-72. doi: 10.1084/jem.67.6.953.
Following the intraperitoneal inoculation of mice with large doses of epidemic influenza virus (50,000 to 1 million intranasal M.L.D.) it can be recovered from the lungs in high concentration, and pulmonary lesions of moderate extent may be observed. The virus reaches its highest titer in the lungs 48 to 72 hours after intraperitoneal injection and may persist for 10 days. Virus may be recovered from the blood in the first 24 hours, but is readily detected in the omentum and peritoneum for 5 to 6 days. Mice which as a result of the intraperitoneal injection of virus show a high concentration of virus in the lungs do not die but become solidly immune to intranasal infection. Moreover, as early as 24 to 48 hours after intraperitoneal inoculation of large amounts of virus the animals may exhibit resistance to infection with fatal doses of virus given intranasally. Influenza virus given intravenously to mice is rapidly removed from the blood but persists in the lungs and induces pulmonary lesions. Virus can also be recovered from the liver for several days. With subcutaneous inoculation of influenza virus, however, the virus does not reach the blood or lungs in detectable amounts although the regional lymph nodes may yield considerable quantities of the agent. A brief consideration is presented of the mechanisms of infection and resistance which may be involved.
给大量流感病毒（50000 至 100 万鼻腔 M.L.D.）腹腔接种的小鼠，可从肺部高度浓缩地回收病毒，并且可能观察到中等程度的肺部病变。病毒在腹腔接种后 48 至 72 小时达到肺部的最高滴度，并可能持续 10 天。病毒可能在首次 24 小时内从血液中回收，但在网膜和腹膜中可在 5 至 6 天内检测到。由于腹腔内注射病毒而导致肺部病毒浓度高的小鼠不会死亡，但对鼻腔感染具有坚实的免疫力。此外，早在腹腔内接种大量病毒后 24 至 48 小时，动物就可能对鼻腔给予致命剂量的病毒感染表现出抵抗力。静脉内给予小鼠的流感病毒会从血液中迅速清除，但在肺部持续存在并引起肺部病变。病毒也可以从肝脏中持续数天回收。然而，皮下接种流感病毒时，病毒不会以可检测的量进入血液或肺部，尽管区域淋巴结可能产生大量病毒。简要讨论了可能涉及的感染和抵抗机制。
