Yap K L, Ada G L
Scand J Immunol. 1978;7(1):73-80. doi: 10.1111/j.1365-3083.1978.tb00428.x.
Cytotoxic T cells are present in the lungs and the bronchoalveolar washings of mice infected intravenously (i.v.) or intranasally (i.n.) with live influenza A/WSN virus. After i.v. injection, cytotoxic T cell activity in both spleens and lungs reaches a peak at 6 days when the level of infectious virus recovered from the lungs falls sharply and the mice do not die. If a lethal dose of virus is given intranasally, very high levels of virus appear rapidly in the lungs, and the development of lung consolidation follows slightly behind the appearance of cytotoxic T cells there. When a non-lethal dose of virus is given intranasally, lower levels of virus are found in the lung and the appearance of cytotoxic T cells is delayed. These results suggest that the cytotoxic T cells play a protective role if the level of virus in the lungs does not reach very high levels. After injection of antithymocyte serum, the subsequent level of cytotoxic T cell activity in the lungs was greatly reduced, suggesting that the T cells recovered in lungs had at an earlier stage been circulating cells. However, splenectomized mice develop high levels of cytotoxic T cell activity, after intranasal infection of mice, indicating that the spleen did not contribute substantially to the T cells recovered in the lungs.
细胞毒性T细胞存在于经静脉内（i.v.）或鼻内（i.n.）接种活甲型流感病毒A/WSN株的小鼠的肺脏和支气管肺泡灌洗液中。静脉内注射后，脾脏和肺脏中的细胞毒性T细胞活性在第6天达到峰值，此时从肺脏中回收的感染性病毒水平急剧下降，小鼠不会死亡。如果经鼻给予致死剂量的病毒，肺脏中会迅速出现非常高的病毒水平，肺部实变的发展略滞后于细胞毒性T细胞在那里的出现。当经鼻给予非致死剂量的病毒时，肺脏中发现的病毒水平较低，细胞毒性T细胞的出现也会延迟。这些结果表明，如果肺脏中的病毒水平未达到非常高的程度，细胞毒性T细胞会发挥保护作用。注射抗胸腺细胞血清后，肺脏中随后的细胞毒性T细胞活性水平大大降低，这表明在肺脏中回收的T细胞在早期是循环细胞。然而，在小鼠经鼻感染后，脾切除的小鼠会产生高水平的细胞毒性T细胞活性，这表明脾脏对在肺脏中回收的T细胞贡献不大。
